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Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

ABSTRACT: Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

PROVIDER: PRJNA827027 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18767724_1.fastq.gz	Fastqsanger.gz
	SRR18767724_2.fastq.gz	Fastqsanger.gz
	SRR18767725_1.fastq.gz	Fastqsanger.gz
	SRR18767725_2.fastq.gz	Fastqsanger.gz
	SRR18767726_1.fastq.gz	Fastqsanger.gz

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Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

Project description:We report the application of high-throughput mRNA sequencing to explore the effect of Id1 depletion in TAMs on gene expression of MC38 cells. Different groups of TAMs were isolated from the subcutaneous tumors in Id1f/f or myeloid cell lineage speciﬁc Id1 deficient mice (Id1-Lyz-KO) . MC38 cells were then mixed with two groups of TAMs and be implanted in C57BL/6J mice. CD45- cells were then isolated from the subcutaneous tumors described above to do the transcriptome sequencing.

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Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

Project description:We report the application of high-throughput mRNA sequencing to explore the effect of Id1 depletion in TAMs on its gene expression . Different groups of TAMs were isolated from the subcutaneous tumors in Id1f/f or myeloid cell lineage speciﬁc Id1 deficient mice (Id1-Lyz-KO) .

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Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

Project description:Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

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Project description:To study the impact of heme-TAMs on the growth, phenotype, and function of cancer cells, we cultured GFP-MC38 cells either alone or mixed with heme-treated macrophages in microwell plates, enabling us to collect large numbers of uniform spheroids for scRNA-seq. We observed that after four days, the size of GFP-MC38 spheroids regressed, and the tumor cells underwent apoptosis and decayed metabolically. In contrast, the presence of heme-TAMs within spheroids prevented apoptosis, supporting the extended growth of metabolically viable spheroids beyond 10 days of culture.

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