Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA108475 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:RNA silencing is a conserved mechanism in which small RNAs trigger various forms of sequence specific gene silencing by guiding Argonaute complexes to target RNAs via base-pairing. RNA silencing is thought to have evolved as a form of nucleic-acid-based immunity to inactivate viruses and transposable elements (TEs). Although the activity of TEs in animals has been thought to be largely restricted to the germline, recent studies have shown that they may also actively transpose in somatic cells, creating somatic mosaicism in animals3. In the Drosophila germline, piRNAs arise from repetitive intergenic elements including retrotransposons by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. Here we show that in Drosophila cultured S2 cells, Argonaute2 (AGO2), an AGO subfamily member of Argonautes, associates with endogenous small RNAs of 20-22 nucleotides in length, which we have collectively named esiRNAs (endogenous siRNAs). esiRNAs can be divided into two groups: one that mainly corresponds to a subset of retrotransposons, and the other that arises from stem-loop structures. esiRNAs are produced in a Dicer-2-dependent manner from distinctive genomic loci, are modified at their 3’ ends and can direct AGO2 to cleave target RNAs. Depletion of Dicer2 or AGO2 in S2 cells coincided with higher levels of retrotransposon transcripts. Together, our findings indicate that in Drosophila, different types of small RNAs and Argonautes are utilized to repress retrotransposons in germline and somatic cells. Keywords: AGO2, small RNA 454 sequencing - high throughput
2008-05-08 | GSE10277 | GEO
Project description:FLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)
| PRJNA114895 | ENA
Project description:Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
| PRJNA107113 | ENA
Project description:Drosophila melanogaster small RNAs in ovaries
| PRJNA215819 | ENA
Project description:modENCODE RNA-Seq of Drosophila BG3 small RNAs
| PRJNA119271 | ENA
Project description:modENCODE RNA-Seq of Drosophila Kc167 small RNAs
| PRJNA119891 | ENA
Project description:Small RNAs from third instar Drosophila melanogaster males
| PRJNA262549 | ENA
Project description:High-throughput sequencing of small RNAs from Drosophila
| PRJNA100223 | ENA
Project description:High throughput seqeuncing of small RNAs (PAGE isolated from total RNA or Argonaute immunoprecipitates) from Drosophila melanogaster using the Illumina platform. Adapter ligation requires 5' monophosphate and 3' OH. Full analysis of all libraries in this set is published (Czech B. et al. 2008), leading to the description of endogenous siRNAs in flies. Keywords: Solexa sequences
2008-04-30 | GSE11086 | GEO
Project description:Small RNAs from Drosophila melanogaster WT and Tailor mutant S2 cells and ovaries
| PRJNA281580 | ENA