Project description:We develop a system for bidirectional epigenetic editing (CRISPRai), in which orthogonal activating (CRISPRa) and repressive (CRISPRi) perturbations are applied simultaneously to multiple loci the same cell. We perform ATAC-seq on CRISPRi perturbed Jurkat T cells to investigate chromatin accessibility changes upon perturbation of individual regulatory elements.
Project description:ATAC-seq analysis was performed in a T-ALL cell line (Jurkat) with FKBP knock-in at MYB locus to analyze chromatin accessibility after dTAG-mediated depletion.
