Project description:Jurkat cell lines were generated to stably express CD46 protein expressing either cytoplasmic tail 1 or 2 (BC1 or BC2). The transcription profile of these unactivated stable lines were compared with unactivated Jurkat cells.
Project description:Vitamin K1 (VK1), an essential nutrient related to coagulation, has been proved to have anticancer properties in various cancer cells. However, little is known about the effects of VK1 on hematologic malignancies. The aim of the study was to evaluate the cytotoxic effects of VK1 on acute T-cell lymphocytic leukemia Jurkat cells (Jurkat T cells). In addition, VK1 induced changes of gene expression in Jurkat T cells were also observed.
Project description:ATAC-seq analysis was performed in a T-ALL cell line (Jurkat) with FKBP knock-in at MYB locus to analyze chromatin accessibility after dTAG-mediated depletion.
Project description:We develop a system for bidirectional epigenetic editing (CRISPRai), in which orthogonal activating (CRISPRa) and repressive (CRISPRi) perturbations are applied simultaneously to multiple loci the same cell. We perform ATAC-seq on CRISPRi perturbed Jurkat T cells to investigate chromatin accessibility changes upon perturbation of individual regulatory elements.