Effect of Rosa roxburghii fruit extracts to gene expression of mesenchymal stem cells
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1089990 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28395639_1.fastq.gz | Fastqsanger.gz | |||
| SRR28395639_2.fastq.gz | Fastqsanger.gz | |||
| SRR28395640_1.fastq.gz | Fastqsanger.gz | |||
| SRR28395640_2.fastq.gz | Fastqsanger.gz | |||
| SRR28395641_1.fastq.gz | Fastqsanger.gz |
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Project description:Here we found Rosa roxburghii fruit extracts effectively increase TERT expression and telomerase activity in cultured human mesenchymal stem cells. Both Rosa roxburghii fruit extracts by freeze drying and spray drying methods increase the activity of telomerase. Rosa roxburghii fruit freeze drying extracts is able to reduce reactive oxygen species levels, enhance SOD activity and resistance to oxidative stress, and reduce DNA damage caused by oxidative stress or radiation. Rosa roxburghii fruit extracts promoted cell proliferation, improved senescent cell morphology, delayed replicative cellular senescence, attenuated cell cycle supressors and alleviated the senescence-associated secretory phenotype. Transcriptome and metabolic profilings found that Rosa roxburghii fruit extract promote cell proliferation and DNA repair pathways, decreased triglycerides as well. Overall, we provided a theoretical basis for the application of Rosa roxburghii fruit as an anti-aging natural product.
2024-04-01 | GSE261987 | GEO
Project description:Rosa roxburghii Tratt belongs to the Rosaceae family, and the fruit is flavorful, economic, and high nutritious, providing health benefits. MYB proteins play key roles in R. roxburghii’ development and fruit quality. However, the available genomic and transcriptomic information are extremely deficient. Here, a normalized cDNA library was constructed using five tissues, stem, leaf, flower, young fruit, and mature fruit, with three repetitions, and sequenced using the Illumina HiSeq 3000 platform. De novo assembly was performed, and 470.66 million clean reads were obtained. In total, 63,727 unigenes, with an average GC content of 42.08%, were determined and 59,358 were annotated. In addition, 9,354 unigenes were assigned the Gene Ontology category, and 20,202 unigenes were assigned to 25 Eukaryotic Ortholog Groups. Additionally, 19,507 unigenes were classified into 140 pathways of the Kyoto Encyclopedia of Genes and Genomes database. Using the transcriptome, 163 unigenes associated with MYB were detected. Among these genes, there were total 75 genes which strikingly expressed in various tissues, including 10 R1 MYB, 42 R2R3 MYB, 1 R1R2R3 MYB, 3 MYB and 19 atypical MYB-like proteins. The expression levels of 12 MYB genes randomly selected for qRT-PCR analysis were consistent with the RNA-seq results. A total of 37,545 microsatellites were detected, with an average EST-SSR frequency of 0.59 (37,545/63,727). This transcriptome data will be valuable for identifying genes of interest and studying their expression and evolution.
2019-02-21 | GSE122014 | GEO
Project description:Rosa roxburghii Transcriptome or Gene expression
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Project description:Hyperlipidemia (HLP), an insidious threat to human health in modern society, can deteriorate and lead to various other metabolic diseases. This study focuses on transcriptomic analysis of liver samples from C57BL/6 mice, which were divided into the following groups: control group (C), hyperlipidemia model group (M), and three groups treated with flavonoids from Rosa roxburghii—quercetin at 200 mg/kg (QU), myricetin at 75 mg/kg (MY), and kaempferol at 100 mg/kg (KA). Except for the control group, all other groups were maintained on a high-fat diet for 6 weeks. To investigate the commonalities among the three flavonoid components from Rosa roxburghii, differentially expressed genes (DEGs) between each treatment group and the model group were screened to identify common expression genes.
2024-07-12 | GSE271599 | GEO
Project description:The study investigates the effects of myrobalane fruit extracts, essential in Asian traditional medicine and notably part of the Triphala formulation, on human cell models. The complexity of these botanical preparations suggests a multi-target mode of action, making it difficult to identify specific active ingredients. The in vitro study revealed that, beyond their antioxidant properties, myrobalane fruit extracts modulate tryptophan metabolism and affect immunobiochemical and cytoprotective signaling pathways in a dose-dependent manner. Integrated transcriptome analysis of treated cells showed impacts on immune response pathways, oxidative stress, and detoxification processes. Specifically, a synergistic activation of the endogenous antioxidant response was observed in liver epithelial cells treated with a combination of the three fruit extracts. These findings highlight the modulation of various signaling pathways and cellular processes, underpinning the complex multi-target effects of myrobalane fruit extracts. Despite being limited to in vitro data, this study enhances the understanding of the mode of action of these botanical mixtures.
2025-03-06 | GSE273987 | GEO
Project description:Myrobalane fruit extracts modulate immunobiochemical pathways in vitro
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Project description:Fructus Rosae Roxburghii (FRR) has been considered as edible and medicinal fruit possessing antiatherosclerotic effect, but the mechanism is still unclear. Hyperlipidemia (HLP) is material basis for atherosclerosis (AS) formation. Under FRR action, TC, TG, LDL, HDL and ASI in serum were regulated to control level. Differentially expressed proteins in liver were analyzed by using TMT labeling and LC-MS/MS for better understanding the effect and molecular mechanism of FRR on diet-induced hyperlipidemic mice. In total, 4460 proteins were quantified, of which 469 proteins showed dramatic changes between each group. According to molecular functions, 25 differentially co-expressed proteins were divided into five categories: substance metabolism, energy transformation and signal transduction, transcription and translation, immune defense. 15 key proteins involved lipids metabolism, which were identified as Cyp7a1, Cyp3a11, Tm7sf2, COAT2, CSAD, RBP3, Lpin1, Dhrs4, Aldh1b1, GK, Acot 4, TSC22D1, PGFS, EHs, GSTM1. Their altered expression suggested that FRR could maintain the metabolism homeostasis by regulating the metabolism of fatty acids, biosynthesis of BAs and steroids, and production of lipid peroxides (LPOs) in mice. It’s first time potential antiatherosclerotic mechanism of FRR regulating blood lipids was explored from protein level, which is of great significance to explore new drug targets for AS.
2021-01-04 | PXD019962 | Pride