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RNA-seq analysis of Sphingosine 18:1 treated mice bone marrow-derived macrophages

ABSTRACT: RNA-seq analysis of Sphingosine 18:1 treated mice bone marrow-derived macrophages

PROVIDER: PRJNA1090453 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28419702_1.fastq.gz	Fastqsanger.gz
	SRR28419702_2.fastq.gz	Fastqsanger.gz
	SRR28419703_1.fastq.gz	Fastqsanger.gz
	SRR28419703_2.fastq.gz	Fastqsanger.gz
	SRR28419704_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of Sphingosine 18:1 treated mice bone marrow-derived macrophages

Project description:Purpose: Study of the influence of Sphingosine 18:1 in macrophages Method: 2μM Sphingosine 18:1 was added to BMDMs for 6 hours, solvent was added as control. mRNA profiles were generated by deep sequencing. Results: Several signaling pathways were changed after Sphingosine administration, including hypoxia-related signal pathway. RT-quantitive PCR was performed to validate the expression of Epas1 and its downstream target genes, results showed that Sphingosine 18:1 significantly inhibits Epas1 expression.

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