Project description:Lychee (Litchi chinensis Sonn.) is a popular fruit worldwide. Lychee downy blight, caused by Peronophythora litchii (P. litchii), is one of the major diseases in lychee. In this study, we conducted a P. litchii infection experiment on the lychee leaves of GW and SFZ, and analyzed the mRNA in the treated leaves using transcriptome RNA sequencing technology. Our research will provide a preliminary basis for the prevention and control of lychee downy blight.
Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
Project description:To understand difference between SFZ cells and costal chondrocytes, total RNAs from SFZ cells and costal chondrocytes were analyzed by microarray.
Project description:Purpose: To demonstrate Runx3's association with organizing the extracellular matrix in SFZ/DZ chondrocytes. Method: RNA samples were collected from SFZ/DZ chondrocytes of 5-day-old Col2a1-Cre;Runx3fl/fl and Runx3fl/fl mice. Results: 18 genes were up- or down-regulated by more than 2-fold in the Runx3 knockout in SFZ. Conclusions: However, genes organizing the extracellular matrix were down-regulated in Runx3 cKO DZ chondrocytes.
Project description:Purpose: To demonstrate Runx3's association with organizing the extracellular matrix in SFZ chondrocytes. Method: Fragment DNA samples were collected by using Anti-FLAG or IgG, from SFZ chondrocytes treated with lipofection of RUNX3-FLAG Results: 21,730 raw peaks met the peak calling criterion. Conclusions: By GO analysis, the extracellular structure organization and collagen fibril organization terms were the most significantly enriched.
Project description:We compared gene expression profiles of SFZ and deep AC of articular cartilage through laser microdissection (LMD) using adhesive tape, linear amplification of mRNA, and mRNA-seq analysis. gene expression profiles of SFZ and deep AC obtained from the proximal tibia of two adult rats