Project description:Cilia and flagella serve as cellular antennae that translate sensory information into cellular responses. In sperm, a single chemoattractant molecule triggers signaling events that evoke a Ca2+ response and chemotactic steering. Key components of the chemotactic signaling pathway from sperm of the sea urchin Arbacia punctulata were investigated. Shotgun proteomics employing SDS-PAGE LC-MS/MS and MudPIT provided the proteome inventory of sperm flagella.
Project description:A total of 132 differentially expressed genes (DEGs) regulated by the RcsB have been identified, of which 114 were up-regulated and 18 were down-regulated. Analyses of the GO annotation and KEGG enrichment led us to focus on the role of RcsB in the inhibition of chemotactic behavior, flagella biosynthesis and bacterial infection in Y. enterocolitica. Overall, the information provided in this study complements our understanding of Rcs phosphorelay in the regulation of Y. enterocolitica pathogenicity, and, simultaneously, provides clues to additional roles of the Rcs system in other members of family Enterobacteriaceae.
Project description:The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for systemic dissemination. We report that, upon T. gondii infection, macrophages initiate expression of transcription factors normally attributed to DCs, upregulate the expression of Ccr7 with a chemotactic response, and perform systemic migration when adoptively transferred into mice. We identify the parasite effector GRA28, which is secreted into the host cell nuclei, as driving the chemotactic migratory activation of parasitized macrophages. To gain insight about the molecular role played by GRA28 in this phenomenon, we characterized its interactome.
2022-11-02 | PXD032360 | Pride
Project description:Natural bacterial community chemotactic to the polysaccharide laminarin
Project description:We extracted RNA from NC and KO-Toe1 differentiated C17.2 cells for high-throughput transcriptomic sequencing. In differentiated C17.2 cells, Toe1 primarily affected biological functions including peptidase activity, chemotactic factors, the ECM, and the TNF signaling pathway.
Project description:In this study, we investigated the functional role of NLRP2 in proximal tubular epithelial cells by overexpressing it in human conditionally immortalized cells obtained from urine of a healthy subject. We found that NLRP2 markedly upregulates proinflammatory and chemotactic responses as well as downregulates interferon-mediated signaling pathways.