Project description:Factorial Microarray Analysis of Zebra Mussel (Dreissena polymorpha) Adhesion Process under the Impact of Multiple Environmental Factors The expression profiles of the zebra mussel byssus unique genes in our cDNA microarray can be influenced by multiple factors. Three environmental factors plus adhesion status were considered as four main factors in this study.
2009-07-10 | GSE16397 | GEO
Project description:Byssus adhesion in golden mussel
Project description:Mussels synthesize an interesting class of biological materials with unique properties to adhere onto virtually any solid surface. Inter- and cross-species analyses have revealed that mussel byssus fabrication is influenced by environmental factors. In this study, proteins expressed specifically byssus and in the byssal-producing organ (foot) were examined for two mussel species from the Mytilidae family using liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-MS/MS). The main goal was to describe which proteins are exclusively expressed in the mussel's foot gland, which is responsible for byssus secretion. Proteins uniquely found in the foot samples have been found by comparing to a control tissue (mantle). The overlap with proteins found in byssus samples were also investigated. A restricted list of these proteins have been highlighted as byssus-related, believed to contribute to the unique properties of byssus, such as securing adhesion and toughness, and plasticity based on environmental factors (including promoter regulation). This work may provide further directions on thread biofabrication and additional information on its regulation, as well as contribute to a more complete description of the mussel proteome.
Project description:A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression
Project description:Factorial Microarray Analysis of Zebra Mussel (Dreissena polymorpha) Adhesion Process under the Impact of Multiple Environmental Factors The expression profiles of the zebra mussel byssus unique genes in our cDNA microarray can be influenced by multiple factors. Three environmental factors plus adhesion status were considered as four main factors in this study. Two different levels in each factor were created, therefore, a 2x2x2x2 factorial experimental design was made by sixteen treatment groups with four biological replicates in each group. There were 32 dual-channel microarray slides included in this study and the effects of main factors on gene expression profiles as well as the two-way, three-way, and four-way interactions of the main factors were analyzed. Factors: temperature, water agitation, oxygen level, and adhesion status
