Project description:Solanum stoloniferum is a wild tuber-bearing species belonging to Solanaceae family. The complete chloroplast genome of S. stoloniferum was constituted by de novo assembly using a small amount of whole genome sequencing data. The chloroplast genome of S. stoloniferum was the circular DNA molecule with a length of 155,567?bp and consisted of 86,007?bp of large single copy, 18,374?bp of small single copy, and 25,593?bp of a pair of inverted repeat regions. A total of 158 genes were annotated including 105 protein-coding genes, 45 tRNA genes, and eight rRNA genes. Maximum likelihood phylogenetic analysis with 25 Solanaceae species revealed that S. stoloniferum is the most closely grouped with S. tuberosum.