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Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome III

ABSTRACT: Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome III

PROVIDER: PRJNA1096332 | ENA |

REPOSITORIES: ENA

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Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome III

Project description:Individuals with Down syndrome have a increased likelihood of suffering from inflammatory and autoimmune conditions, due to the presence of four interferon receptors on chromosome 21. Transcription of genes plays a key role in the interferon response, and the Mediator kinases, CDK8 and CDK19, are essential in potentiating this transcription. Given the role CDK8/19 play in the interferon response, and the fact that selective inhibitors of these kinases exist (such as Cortistatin A), it is possible that targetting Mediator kinase activity may alleviate symptoms of chronic inflammation in Down syndrome.

2025-02-04 | GSE263239 | GEO

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

Project description:Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

| PRJNA910594 | ENA

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome II

Project description:Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome II

| PRJNA1049466 | ENA

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome I

Project description:Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome I

| PRJNA1049467 | ENA

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

2023-09-12 | GSE220652 | GEO

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome I

2025-01-01 | GSE249548 | GEO

Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome II

2024-09-01 | GSE249549 | GEO

Transcriptional responses to interferon require Mediator kinase-dependent pause release and mechanistically distinct functions of CDK8 and CDK19

Project description:Transcriptional responses to interferon require Mediator kinase-dependent pause release and mechanistically distinct functions of CDK8 and CDK19.

2019-07-15 | GSE129501 | GEO

Mediator Kinase Complexes Regulates ATOH1-Dependent Intestinal Differentiation

Project description:Cyclin Dependent Kinases CDK8 and CDK19 (Mediator kinase) are regulatory components of the Mediator complex, a highly conserved complex that fine tunes transcriptional output. While Mediator kinase has been implicated in the transcriptional control of key pathways necessary for development and growth, its function in vivo has not been well described. Herein, we report the consequences of complete ablation of both Cdk8/19 on tissue homeostasis. We show that intestinal epithelial specific deletion of Mediator kinase leads to a distinct defect in secretory progenitor differentiation with broad loss of the intestinal secretory cell types. Using a phospho-proteogenomic approach, we show that the Cdk8/19 kinase module interacts with and phosphorylates components of the chromatin remodeling complex Swi/Snf in intestinal epithelial cells. Genomic localisation of Swi/Snf and Mediator shows Cdk8/19-dependent genomic binding at distinct super-enhancer loci within key lineage specification genes, including the master regulator of secretory differentiation ATOH1. Using CRISPRi/CRISPRa, we identify a distinct Mediator- Swi/Snf bound enhancer element that is necessary and sufficient for ATOH1 expression in a Mediator-kinase dependent manner. As such, these studies uncover a newly described transcriptional mechanism of ATOH1-dependent intestinal cell specification that is dependent on the coordinated interaction of the chromatin remodeling complex Swi/Snf and Mediator complex.

2023-03-10 | PXD030212 | Pride

The Cdk8 kinase module regulates Mediator-RNA polymerase II interaction

Project description:The Cdk8 kinase module (CKM) is a dissociable part of the coactivator complex Mediator that regulates RNA polymerase II (Pol II transcription. The CKM has negative and positive functions in gene transcription that remain poorly understood at the mechanistic level. Here, we prepare recombinant CKM from the yeast Saccharomyces cerevisiae and show that it binds core Mediator (cMed) to sterically inhibit cMed binding to the Pol II preinitiation complex (PIC) in vitro. We further show that the Cdk8 kinase activity of CKM counteracts CKM-cMed interaction,thereby releasing CKM and enabling Mediator to bind the PIC. Finally, we report that the kinase activity of Cdk8 is required for gene activation during heat shock in vivo, but not under steady state growth conditions. These results converge with previous literature on a model for CKM function. In this model, CKM negatively regulates Mediator function at upstream activating sequences by preventing Mediator binding to the PIC at the promoter. During gene activation, Cdk8 kinase activity may release Mediator and allow its binding to the PIC, thereby stimulating transcription initiation and accounting for the positive function of CKM.

2021-06-16 | GSE161140 | GEO

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