Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization

ABSTRACT: Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization

PROVIDER: PRJNA1096725 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Endometrial gland impacts on stromal cell decidualization

Project description:Uterine glands and, by inference, their secretions impact uterine receptivity, blastocyst implantation, stromal cell decidualization, and placental development. Changes in gland function across the menstrual cycle are impacted by steroid hormones, estrogen and progesterone, as well as stroma-derived factors. Using an endometrial epithelial organoid (EEO) system, transcriptome and proteome analyses identified distinct responses of the EEO to steroid hormones and prostaglandin E2 (PGE2). Notably, steroid hormones and PGE2 modulated the basolateral secretion of EEO proteins, where cystatin C (CST3) was significantly increased by progesterone and PGE2. CST3 treatment of decidualizing stromal cells significantly decreased the decidualization markers PRL and IGFBP1. The attenuation of stromal cell decidualization via CST3 suggests a role for uterine gland-derived proteins in controlling the extent of decidualization. These findings provide evidence that uterine gland-derived factors directly impact stromal cell decidualization, which has strong implications for better understanding pregnancy establishment and female fertility in humans.

2023-10-11 | PXD034357 | Pride

Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization

Project description:We developed an in vitro model in which primary human endometrial stromal cells (HESCs) were induced to differentiate through treatment with MPA and 8-Br-cAMP. SiRNA-mediated knockdown of METTL3 was performed on HESCs, 6 h prior to treatment of 8-Br-cAMP and MPA.

2024-04-14 | GSE263330 | GEO

Endometrial extracellular vesicle decidualization

Project description:Proper decidualization is vital in preparation for a potential embryo receptivity, placentation, menstrual health and subsequent endometrial regeneration. Given the importance of extracellular vesicles (EVs) in intercellular communication, and recently in embryo implantation and indicators of menstrual cycle and fertility, we investigated their role during decidualization. Overall, this study provides an insight into distinct variation in sEV composition depending upon the level of decidualization of endometrial stromal cells, with the signaling potential to coordinate endometrial health ranging from embryo implantation, facilitating placentation and subsequent endometrial regeneration.

2021-11-17 | PXD026342 | Pride

Endometrial gland impacts on stromal cell decidualization

2022-06-06 | GSE205084 | GEO

Transcription profiling time series of the cAMP-induced decidualization of human endometrial stromal cells

Project description:Timecourse of cAMP-induced decidualization of endometrial stromal cells.<br><br>Note that files GSM5962.txt and GSM5965.txt as imported from GEO are identical.

2007-12-13 | E-GEOD-403 | biostudies-arrayexpress

Reduced SCRIB expression compromises endometrial stromal cells decidualization

Project description:To identify the molecular pathway regulated by Scribble (SCRIB) in primary hunan endometrial stromal cells (ESCs) decidualization, high-throughput RNA-seq was performed to analyze the transcriptome profile in si-Ctrl or si-SCRIB transfected decidualized ESCs (dESCs).

2021-12-31 | GSE164331 | GEO

Endometrial gland impacts on stromal cell decidualization

Project description:Endometrial gland impacts on stromal cell decidualization

| PRJNA843696 | ENA

Decidualization of human endometrial stromal fibroblasts is a multi-phasic process involving distinct transcriptional programs

Project description:We sequenced mRNA from endometrial stromal fibroblasts and decidual stromal cells after 3 day and 8 days decidualization treatment

2018-10-25 | GSE114296 | GEO

GATA2 and progesterone receptor interaction in endometrial stromal cells undergoing decidualization [ChIP-Seq]

Project description:The zinc-finger transcription factor GATA2 has been shown to be important for endometrial stromal cell decidualization in early pregnancy in mice and humans. Progesterone and its receptor PGR is also critical during decidualization but its interaction with GATA2 in regulating genes and pathways necessary for decidualization in human endometrium are unclear. Human endometrial stromal cells were isolated from 5 premenopausal women for primary cell culture. The cells underwent in vitro decidualization (IVD) or vehicle (Veh) treatment for 10 days. RNA-sequencing (RNA-seq) was performed to compare gene expression profiles (n=3) and chromatin immunoprecipitation followed by sequencing (ChIP-seq) using an antibody against GATA2 (n=2) was performed to examine binding to target genes in the Veh and IVD cells. A public PGR ChIP-seq dataset (GSE69539) was mined to identify PGR-binding regions in IVD-treated human endometrial cells.

2020-02-22 | GSE145673 | GEO

GATA2 and progesterone receptor interaction in endometrial stromal cells undergoing decidualization [RNA-Seq]

2020-02-22 | GSE145671 | GEO