Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Experiment Overall Design: Interscapular brown adipose tissue and epididymal white adipose tissue was carefully dissected from 3 male C57Bl/6 mice. These samples were profiled independently using Affymetrix mouse 430_2 gene arrays, representing 3 biological replicates for each brown and white adipose tissues.
Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Keywords: tissue comparison from mice
Project description:This project includes proteomic data of brown adipose tissue from high-fat diet-induced obese mice versus wild-type mice. WTB reprents the brown adipose tissue from wild-type mice.DIOB reprents the brown adipose tissue from diet-induced obesity mice.
Project description:This project includes proteomic data of brown adipose tissue from mice modeled with Cuprizone 300mg/Kg/Day for one month versus wild-type mice. CB represents the brown adipose tissue from wild type mice. MB represents the brown adipose tissue from mice treated with cuprizone.
Project description:Brown adipose tissue is a specialized fat tissue involved in heat generation by using a process termed thermogenesis. This ability to convert nutrient energy into heat sets brown adipocytes apart from the more common type of white adipocytes, which are mainly involved in energy storage. Brown adipose depots occur as a large interscapular depot in mice. Brown adipocytes are multilocular, i.e. contain many small lipid-filled vacuoles and uniquely express the uncoupling protein 1. In this study, the gene expression patterns in brown adipose tissue samples of young, healthy mice was analyzed.
Project description:Different human adipose tissue depots may have functional differences. Subcutaneous human adipose tissue has been extensively studied, but less is known about other depots. Perithyroid (PT) adipose tissue contains not only white adipocytes but also brown adipocytes. The aim of this study was to compare the expression of brown adipocyte containing perithyroid adipose tissue with s.c. adipose tissue.role in the development of obesity. Expression profiling of adipose tissue may give insights into mechanisms contributing to obesity and obesity-related disorders.
Project description:This study aims to conduct a combined analysis of differentially expressed genes in the transcriptome and proteome of brown adipose tissue from cold-tolerant rats, exploring potential biomarkers and therapeutic targets for cold adaptation. Male SD rats underwent intermittent cold exposure, with control animals maintained at room temperature. After 21 days, brown adipose tissue samples were collected following anaesthesia with 20% urethane. High-throughput mRNA transcriptome sequencing identified differentially expressed genes within brown adipose tissue.
Project description:Adipose tissue is the major depot for energy storage. Recent studies have shown that at least three types of adipocytes can be distinguished depending on their anatomical locations : 1) The classic brown adipocytes, i.e., brown adipose tissue (BAT); 2) The 'brite' (brown-in-white) adipocytes, i.e. inguinal white adipose tissue (iWAT); 3) The 'true' white adipocytes, i.e., epididymal white adipose tissue (eWAT). Two strains of mice (SV129 and C57BL/6J) were used in this study. SV strain is resistant to obesity and latter is prone to obesity. Pre-adipocyte cells were isolated from subcutaneous tissue (iWAT) to create four groups of cell cultures per strain of mouse.
Project description:Next generation sequencing was used to compare transcriptome profiling in brown adipose tissue from Ctrl and BKO mice with mature brown adipose tissue specific deletion of BSCL2. Using an optimized data analysis workflow, we identified 13,525 transcripts in brown adipose tissue. 243 genes were differentially expressed with a fold change ≥1.5 and Padj value <0.05. Only 99 genes showed differential expression between the Ctrl and BKO brown fat, with a fold change ≥2.0 and Padj value <0.05. Results provide insights into the processes and signaling pathways BSCL2-mediated in brown adipose tissue.
