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Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans

ABSTRACT: Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans

PROVIDER: PRJNA1099906 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28673949.fastq.gz	Fastqsanger.gz
	SRR28673950.fastq.gz	Fastqsanger.gz
	SRR28673951.fastq.gz	Fastqsanger.gz
	SRR28673952.fastq.gz	Fastqsanger.gz
	SRR28673953.fastq.gz	Fastqsanger.gz

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Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans

Project description:Primary microRNA (pri-miRNA) transcripts are processed by a protein complex called the Microprocessor comprised of the ribonuclease Drosha and its RNA binding partner DGCR8/Pasha. We sequenced small RNAs from animals containing a mutation in the WW domain of C. elegans pash-1. We found that these mutants have a modest but widespread reduction in miRNA levels when grown at 20˚C, which is further enhanced when grown at 25˚C. The results demonstrate a requirement for the WW domain in processing miRNAs in C. elegans.

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