Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA110013 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiles of sandwich-cultured primary human hepatocytes exposed to 5 mM and 10 mM acetaminophen were used in a parallelogram approach in order to compare gene expression responses between rat and human using in vitro cellular models, hepatocytes, and between rat in vitro and in vivo. Experiment Overall Design: Samples were retrieved from acetaminophen treated human hepatocyte cultures from 5 individuals (5 biological replicates). Experiment Overall Design: Human hepatocytes from each replicate were treated with 0, 5, and 10 mM acetaminophen for 24 h. Experiment Overall Design: This resulted in (3x5) 15 dual channel arrays on which control (0 mM acetaminophen) and reference samples (0, 5, and 10 mM acetaminophen) were hybridized.
2009-01-14 | E-GEOD-13430 | biostudies-arrayexpress
Project description:Gene expression profiles of sandwich-cutlured primary rat hepatocytes exposed to 5 mM and 10 mM acetaminophen were used in a parallelogram approach in order to compare gene expression responses between rat and human using in vitro cellular models, heaptocytes, and between rat in vitro and in vivo Experiment Overall Design: Samples were retrieved from acetaminophen treated rat hepatocyte cultures from 3 rats (3 biological replicates). Rat hepatocytes from each replicate were cultured in two culture conditions, each treated with 0, 5, and 10 mM acetaminophen for 24 h. This resulted in (3 rats x 2 culture conditions x 3 doses) 18 dual channel arrays on which control (0 mM acetaminophen) and reference samples (0, 5, and 10 mM acetaminophen) were hybridized
2009-01-14 | E-GEOD-13465 | biostudies-arrayexpress
Project description:Acetaminophen-induced gene expression profiles in sandwich-cultured primary rat hepatoctyes
| PRJNA109991 | ENA
Project description:Small hepatocyte progenitor cells (SHPCs) can be derived from primary adult human hepatocytes on mouse embryonic fibroblasts (MEFs). SHPCs derived and cultured on MEFs retain their differentiated morphology and gene expression profiles over several passages. SHPCs in MEF co-culture metabolize acetaminophen to all of its major metabolites at rates equal to mature adult primary human hepatocytes.
2020-09-11 | GSE157791 | GEO
Project description:The frequent use of rodent hepatic in vitro systems in pharmacological and toxicological investigations challenges extrapolation of in vitro results to the situation in vivo and interspecies extrapolation from rodents to humans. The toxicogenomics approach may aid in evaluating relevance of these model systems for human risk assessment by direct comparison of toxicant-induced gene expression profiles and infers mechanisms between several systems. In the present study, acetaminophen (APAP) was used as a model compound to compare gene expression responses between rat and human using in vitro cellular models, hepatocytes, and between rat in vitro and in vivo. Comparison at the level of modulated biochemical pathways and biological processes rather than at that of individual genes appears preferable as it increases the overlap between various systems. Pathway analysis by T-profiler revealed similar biochemical pathways and biological processes repressed in rat and human hepatocytes in vitro, as well as in rat liver in vitro and in vivo. Repressed pathways comprised energy-consuming biochemical pathways, mitochondrial function, and oxidoreductase activity. Conclusion: the present study is the first that used a toxicogenomics-based parallelogram approach, extrapolating in vitro to in vivo and interspecies, to reveal relevant mechanisms indicative of APAP-induced liver toxicity in humans in vivo. expression profiles of sandwich-cultured primary human hepatocytes exposed to 5 mM and 10 mM acetaminophen were used in a parallelogram approach in order to compare gene expression responses between rat and human using in vitro cellular models, hepatocytes, and between rat in vitro and in vivo. Keywords: Toxicogenomics, dose response
2009-01-01 | GSE13430 | GEO
Project description:To identify TJP1-interacting proteins in primary hepatocytes cultured in collagen sandwich configuration (SD+) in vitro
2020-02-17 | PXD016917 | Pride
Project description:Micro RNA content of extracellular vesicles/exosomes may serve as a marker of certain pathophysiological conditions. We report differential expression of micro RNA in exosomes isolated from mouse blood or medium of cultured primary mouse hepatocytes versus parental hepatocytes or whole liver. Studies included exosomes from blood of mice or medium of cultured primary hepatocytes with acetaminophen toxicity as well as a potentially therapeutic cytokine with acetaminophen.
2019-12-02 | GSE77554 | GEO
Project description:The frequent use of rodent hepatic in vitro systems in pharmacological and toxicological investigations challenges extrapolation of in vitro results to the situation in vivo and interspecies extrapolation from rodents to humans. The toxicogenomics approach may aid in evaluating relevance of these model systems for human risk assessment by direct comparison of toxicant-induced gene expression profiles and infers mechanisms between several systems. In the present study, acetaminophen (APAP) was used as a model compound to compare gene expression responses between rat and human using in vitro cellular models, hepatocytes, and between rat in vitro and in vivo. Comparison at the level of modulated biochemical pathways and biological processes rather than at that of individual genes appears preferable as it increases the overlap between various systems. Pathway analysis by T-profiler revealed similar biochemical pathways and biological processes repressed in rat and human hepatocytes in vitro, as well as in rat liver in vitro and in vivo. Repressed pathways comprised energy-consuming biochemical pathways, mitochondrial function, and oxidoreductase activity. Conclusion: the present study is the first that used a toxicogenomics-based parallelogram approach, extrapolating in vitro to in vivo and interspecies, to reveal relevant mechanisms indicative of APAP-induced liver toxicity in humans in vivo. Gene expression profiles of sandwich-cutlured primary rat hepatocytes exposed to 5 mM and 10 mM acetaminophen were used in a parallelogram approach in order to compare gene expression responses between rat and human using in vitro cellular models, heaptocytes, and between rat in vitro and in vivo Keywords: Toxicogenomics, dose response
2009-01-01 | GSE13465 | GEO
Project description:Idelalisib is a phosphatidylinositol 3-delta kinase inhibitor that has shown good efficacy in treating some hematologic malignancies. Rare, but potentially serious liver toxicity was associated with idelalisib use in clinical trials. The objective of this study was to evaluate the idelalisib-induced activation of stress response pathways in human hepatocytes to inform mechanisms of liver injury observed in the clinic Primary human hepatocytes from three donors were sandwich cultured and assayed for global gene expression across 5 concentrations of idelalisib after a 24 h exposure. The most significantly enriched pathway among genes upregulated in response to idelalisib was “Endoplasmic Reticulum Stress”. These data suggest that oxidative stress is a dominant mechanism contributing to liver injury associated with idelalisib.
2022-08-20 | GSE136059 | GEO
Project description:Inferring drug-induced gene regulatory relationships in primary human hepatocytes
| PRJNA140981 | ENA