Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identifying differences in differentiation between adherent and suspension culture for iPSC derived beta cells

ABSTRACT: Identifying differences in differentiation between adherent and suspension culture for iPSC derived beta cells

PROVIDER: PRJNA1101164 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identifying differences in differentiation between adherent and suspension culture for iPSC derived beta cells

Project description:Stage 3 cells from a suspension environment and adherent culture were compared and evaluated through RNA sequencing. RNA sequencing data in heatmap and volcano plot evaluation of core endocrine genes showed that expression levels in adherent cells were lower than those expressed in 3D environment. Several genes that are essential for insulin transcription such as NEUROD1, MAFA, PPARGC1A and NKX2.2 along with INS were expressed with higher transcript levels in bioreactor samples. It was observed that genes usually expressed in earlier stages such as FOXA2 and HNF1b had higher expression levels in adherent culture indicating that 2D environment differentiation might be lagging.

2024-04-26 | GSE264176 | GEO

Expression analysis of doxycycline inducible secondary fibroblasts reprogramming under adherent and suspension conditions

Project description:Samples of adherent and suspension cells undergoing reprogramming were collected at day 0, day2, day6, day15 (with doxycycline) and day25 (without doxycycline).

2012-03-25 | GSE35422 | GEO

Expression analysis of doxycycline inducible secondary fibroblasts reprogramming under adherent and suspension conditions

Project description:Samples of adherent and suspension cells undergoing reprogramming were collected at day 0, day2, day6, day15 (with doxycycline) and day25 (without doxycycline). Reprogramming under adherent and suspension conditions was compared by PCC analysis, non-hierarchical clustering and by functional criteria for differentially expressed genes.

2012-03-25 | E-GEOD-35422 | biostudies-arrayexpress

RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

Project description:Deep sequencing of cDNA of Neisseria gonorrhoeae bacteria that were grown in suspension or adherent to acid-washed glass coverslips, in media where zinc was sequestered or available in excess.

2022-12-12 | GSE191020 | GEO

Differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell lines CAL27 and HSC3

Project description:To investigate the differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell, We compared the difference in gene expression between CAL27 and HSC3 cultured in suspension culture and adherent culture.

2024-05-12 | GSE228889 | GEO

Characterization of a suspension Vero cell line for viral vaccine production

Project description:Vero cells, as approved by the World Health Organization, have been the most commonly used continuous cell line for viral vaccine production over the last 25 years, but their adherent phenotype continues to limit productivity. Adapting to a suspension culture would overcome this restriction and reduce production costs. First, a Vero suspension isolate was obtained and metabolically characterized. Second, RNA sequencing analysis was used to identify differentially expressed genes between adherent and suspension cells, which revealed complete downregulation of adhesion and matrix-associated genes. Additionally, signaling pathways involving Wnt and other tyrosine kinase receptors were identified as potential leads for growth optimization. In particular, supplementation with fibroblast growth factor 2 allowed for a 20% increase in cell density. Finally, a comparative viral productivity assay revealed a 30% increase in poliovirus production in suspension Vero cells compared to adherent cells depending on the serotype, as well as a 140% increase in respiratory syncytial virus production and a 150% increase in yellow fever virus production.This work establishes the potential of the suspension Vero cell line as a new cell platform for viral vaccine production.

2024-06-18 | GSE250417 | GEO

Soybean Suspension culture analysis

Project description:Proteomic analysis of soybean suspension cell extracellular and intracellular protein

2022-02-22 | PXD001895 | Pride

RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

Project description:RNA-sequencing of PEO1 cells grown in suspension (SUS1 and SUS3) was performed to evaluate transcriptional reprogramming during anokis escape. CRISPR/Cas9 screen of PEO1 cells grown in adherent and suspension culture settings for 5 days or 10 days. GeCKO v2 library transduced in PEO1 cell line and cells were selected with puromycin. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).

2018-12-04 | GSE123290 | GEO

Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

Project description:Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

| PRJEB44757 | ENA

RNA-seq data for Huh7 cells in spheroid culture and adherent culture

Project description:Anchorage-independent spheroid cells of HCC possess stemness properties. Huh7 cells are cultured in ultra-low attachment surface plates with serum-free medium. RNA-sequencing (RNA-seq) was applied in spheroid culture cells and adherent culture cells.

2022-06-01 | GSE199940 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data