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ABSTRACT:
PROVIDER: PRJNA1101860 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
2015-04-22 | GSE48671 | GEO
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance. Examination of mRNA transcript abundances in hydrated, dehydrating to 70% RWC and dehydrating to 10% RWC leaf tissues, for each treatment, three biological replicates were included.
2015-04-22 | E-GEOD-48671 | biostudies-arrayexpress
Project description:Gene transcript abundances were analyzed with samples taken from desiccated (10% RWC), rehydrated to 50% RWC (RE50% RWC) and rehydrated to 100% RWC (RE100% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 10207 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
2018-02-22 | GSE66474 | GEO
Project description:Pocillopora damicornis, Pocillopora acuta, Pocillopora verrucosa, Pocillopora eydouxi, Pocillopora meandrina, Pocillopora ligulata, Pocillopora sp. B, Stylophora pistillata, Seriatopora hystrix Raw ezRAD sequence reads for species delineation
| PRJNA386062 | ENA
Project description:The remarkable desiccation tolerance of the vegetative tissues in the resurrection species Craterostigma plantagineum (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall research has greatly advanced thanks to the use of inhibitors affecting the biosynthesis of e.g. cellulose, since they allowed the identification of the compensatory mechanisms underlying habituation. Considering the innate cell wall plasticity of C. plantagineum, the goal of this investigation was to understand whether habituation to the cellulose biosynthesis inhibitors dichlobenil and isoxaben entailed or not identical mechanisms as known for non-resurrection species and to decipher the cell wall proteome of habituated cells. The results showed that exposure of C. plantagineum calli/cells triggered abnormal phenotypes as reported in non-resurrection species. Additionally, the data demonstrated that it was possible to habituate Craterostigma cells to dichlobenil and isoxaben and that gene expression and proteomics did not follow the same trend. Shotgun and gel-based proteomics revealed a common set of proteins induced upon habituation, but also identified candidates solely induced by habituation to one of the two inhibitors. Finally, it is hypothesized that alterations in auxin levels are responsible for the increased abundance of cell wall-related proteins upon habituation.
2022-02-17 | PXD026723 | Pride
Project description:The remarkable desiccation tolerance of the vegetative tissues in the resurrection species Craterostigma plantagineum (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall research has greatly advanced thanks to the use of inhibitors affecting the biosynthesis of e.g. cellulose, since they allowed the identification of the compensatory mechanisms underlying habituation. Considering the innate cell wall plasticity of C. plantagineum, the goal of this investigation was to understand whether habituation to the cellulose biosynthesis inhibitors dichlobenil and isoxaben entailed or not identical mechanisms as known for non-resurrection species and to decipher the cell wall proteome of habituated cells. The results showed that exposure of C. plantagineum calli/cells triggered abnormal phenotypes as reported in non-resurrection species. Additionally, the data demonstrated that it was possible to habituate Craterostigma cells to dichlobenil and isoxaben and that gene expression and proteomics did not follow the same trend. Shotgun and gel-based proteomics revealed a common set of proteins induced upon habituation, but also identified candidates solely induced by habituation to one of the two inhibitors. Finally, it is hypothesized that alterations in auxin levels are responsible for the increased abundance of cell wall-related proteins upon habituation.
2021-11-03 | PXD026733 | Pride
Project description:Report transcritpome analysis of Arabian horses before and after exercise
2019-01-23 | GSE104388 | GEO
Project description:Water shortage is a major factor that harms agriculture and ecosystems worldwide. Plants display various levels of tolerance to water deficit, but only resurrection plants can survive full desiccation of their vegetative tissues. Haberlea rhodopensis, an endemic plant of the Balkans, is one of the few resurrection plants found in Europe. We performed transcriptomic analyses of this species under slight, severe and full dehydration and recovery to investigate the dynamics of gene expression and associate them with existing physiological and metabolomics data. De novo assembly yielded a total of 142,479 unigenes with an average sequence length of 1,034 nt. Among them, 18,110 unigenes were differentially expressed. Hierarchical clustering of all differentially expressed genes resulted in seven clusters of dynamic expression patterns. The most significant expression changes, involving more than 15,000 genes, started at severe dehydration (~20% relative water content) and were partially maintained at full desiccation (<10% relative water content). More than a hundred pathways were enriched and functionally organized in a GO/pathway network at the severe dehydration stage. Transcriptomic changes in key pathways were analyzed and discussed in relation to metabolic processes, signal transduction, quality control of protein and DNA repair in this plant during dehydration and rehydration. Reprograming of the transcriptome occurs during severe dehydration, resulting in a profound alteration of metabolism toward alternative energy supply, hormone signal transduction, and prevention of DNA/protein damage under very low cellular water content, underlying the observed physiological and metabolic responses and the resurrection behavior of H. rhodopensis.
2018-11-20 | GSE109958 | GEO
Project description:RNA-Seq data from the resurrection plant Xerophyta schlechteri during desiccation in three tissues: non-senescent, senescent and pre-senescent during dehydration and rehydration.
2022-12-30 | GSE168229 | GEO