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MRNA profiling in dss1(I) and dss1(V) mutant lines of Arabidopsis thaliana under oxidative stress (hydrogen peroxide)

ABSTRACT: mRNA profiling in dss1(I) and dss1(V) mutant lines of Arabidopsis thaliana under oxidative stress (hydrogen peroxide)

PROVIDER: PRJNA1103187 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28771262_1.fastq.gz	Fastqsanger.gz
	SRR28771262_2.fastq.gz	Fastqsanger.gz
	SRR28771263_1.fastq.gz	Fastqsanger.gz
	SRR28771263_2.fastq.gz	Fastqsanger.gz
	SRR28771264_1.fastq.gz	Fastqsanger.gz

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mRNA profiling in dss1(I) and dss1(V) mutant lines of Arabidopsis thaliana under oxidative stress (hydrogen peroxide)

Project description:The Arabidopsis genome contains two highly homologous DSS1 genes, AtDSS1(I) and AtDSS1(V). This study was designed to identify the functional differences between AtDSS1s, focusing on their potential roles in oxidative stress. We generated single dss1(I) and dss1(V) mutant lines of both Arabidopsis DSS1 genes using CRISPR/Cas9 technology. The sensitivity of homozygous mutant lines with large indels (dss1(I)del25 and dss1(V)ins18) were analyzed to oxidative stress. Plant seedlings were exposed to oxidative stress induced by 10 mM hydrogen peroxide. Transcriptome analysis revealed that the absence of either of DSS1 genes caused alterations in the expression of different genes under stress conditions.

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