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Transcriptome analysis of Streptococcus mutans quorum sensing-induced cells

ABSTRACT: Transcriptome analysis of Streptococcus mutans quorum sensing-induced cells

PROVIDER: PRJNA1103774 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28781388_1.fastq.gz	Fastqsanger.gz
	SRR28781388_2.fastq.gz	Fastqsanger.gz
	SRR28781389_1.fastq.gz	Fastqsanger.gz
	SRR28781389_2.fastq.gz	Fastqsanger.gz
	SRR28781390_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Streptococcus mutans quorum sensing-induced cells

Project description:In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that controls different stress-induced phenotypes. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the CSP-induced stationary phase cells. The data obtained contribute to the understanding of the CSP-induced phenotypes in S. mutans.

2024-04-28 | GSE264670 | GEO

Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.

2010-07-29 | GSE23159 | GEO

Transcriptome analysis of Streptococcus mutans quorum sensing-mediated persisters reveals an enrichment in genes related to stress-defense mechanisms

Project description:Persisters are a small fraction of growth-arrested phenotypic variants that can survive lethal concentrations of antibiotics but are able to resume growth once antibiotics are stopped. Their formation can be a stochastic process or triggered by environmental cues. In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that promotes the formation of stress-induced persisters. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions and isolated the antibiotic persisters by treating the cultures with ofloxacin. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the persisters. RNA-sequencing revealed that triggered persistence was associated with the up-regulation of genes related to several stress-defense mechanisms, notably, multidrug efflux pumps, the arginine deaminase pathway, and the Opu/Opc system. In addition, we showed that inactivation of the VicK kinase of the YycFG essential two-component regulatory system abolished the formation of triggered persisters via the CSP pheromone. These data contribute to the understanding of the triggered persistence phenotype and may suggest new therapeutic strategies for treating persistent streptococcal infections

2023-09-29 | GSE244067 | GEO

Transcriptomic profiles of Streptococcus mutans UA159 grown in the presence or absence of mucins or mucin glycans

Project description:We use high-throughput sequencing to profile the response of oral commensal pathogen Streptococcus mutans to mucins protein polymers (human MUC5B mucins) and soluble mucin glycans (human MUC5B glycans and porcine MUC5AC glycans). We find that mucins and their glycans alter the regulation of dozens of S. mutans genes, specifically downregulating competence-associated quorum sensing genes. The transcriptional responses induced by MUC5B mucins, MUC5B glycans, and MUC5AC glycans are highly correlated.

2021-01-01 | GSE163258 | GEO

Interactions with Commensal or Non-commensal Streptococci Alter Streptococcus mutans Transcriptome and Behaviors

Project description:RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. This revealed a common transcriptome pattern in S. mutans when grown in mixed-species culture, indepenedent of the species identity that S. mutans was cultured with. Additionally, transcriptome changes in the commensal species could also be determined when undergoing competition from S. mutans. RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture or in coculture with Streptococcus sobrinus NIDR 6715, Lactobacillus casei ATCC 4646 or Corynebacterium matruchotii ATCC 14266. These data were compared to previous coculture and quadculture RNA-Seq data with commensal streptococci (GSE209925). These data confirmed a common transcriptome pattern in S. mutans when grown in mixed-species culture with commensal streptococci that is not present with non-commensal streptococci, indepenedent of the species identity that S. mutans was cultured with.

2022-08-03 | GSE209925 | GEO

Transcriptome analysis of Streptococcus mutans biofilms in response to cranberry proanthocyanidins

Project description:The influence of cranberry proanthocyanidins on the transcriptomic responses of Streptococcus mutans during biofilm formation was investigated.

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Transcriptome analysis of Streptococcus mutans biofilms in response to starch and sucrose.

Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose at distinct stages of biofilm development.

2010-05-15 | GSE21831 | GEO

Transcriptome analysis of Streptococcus mutans quorum sensing-mediated persisters reveals an enrichment in genes related to stress-defense mechanisms

Project description:Transcriptome analysis of Streptococcus mutans quorum sensing-mediated persisters reveals an enrichment in genes related to stress-defense mechanisms

| PRJNA1021191 | ENA

ΔfruB effects on the transcriptome of Streptococcus mutans probed by RNA-seq

Project description:Streptococcus mutans, the primary etiologic agent of human dental caries, and a variety of oral Streptococcus and Actinomyces spp. synthesize high molecular mass homopolymers of fructose (fructans) with predominantly β2,1- (inulins) or β2,6-linkages (levans). The ability of S. mutans to degrade fructans contributes to the severity of dental caries. The extracellular product of fruA of S. mutans is an exo-β-D-fructofuranosidase that releases fructose from levan and inulin. Located 70 bp downstream of fruA, fruB encodes a member of the glycoside hydrolase family 32, but the function of FruB has not been established.In this study, the transcriptomic analysis of UA159 and a fruB mutant grown on 0.2% levan revealed differential expression of genes encoding ABC transporters, transcriptional regulators and genes involved in growth and stress tolerance. The results provide new insights into the mechanisms of action by which FruB utilizes homo-polymers of fructose, specifically levan. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen S. mutans.

2022-04-15 | GSE200727 | GEO

Transcriptome analysis of Streptococcus mutans biofilms in response to starch and sucrose.

Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose at distinct stages of biofilm development. RNA was extracted and purified from four replicate samples of each biofilm sample of interest and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.

2010-05-15 | E-GEOD-21831 | biostudies-arrayexpress

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