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Arabidopsis thaliana

ABSTRACT: Transcriptome changes triggered by the synthetic defense elicitors DCA and INA in Arabidopsis thaliana

OTHER RELATED OMICS DATASETS IN: E-GEOD-13833

PROVIDER: PRJNA110511 | ENA |

REPOSITORIES: ENA

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Transcriptome changes triggered by the synthetic defense elicitors DCA and INA in Arabidopsis thaliana

2009-03-20 | GSE13833 | GEO

Transcription profiling of Arabidopsis thaliana seedlings treated with synthetic defense elicitors 3,5-Dichloroanthranilic acid (DCA) and 2,6-Dichloroisonicotinic acid (INA)

Project description:DCA (3,5-Dichloroanthranilic acid) is a newly identified synthetic defense elicitor. To perform a comparative analysis of defense responses triggered by DCA and the structurally related defense inducer INA (2,6-Dichloroisonicotinic acid) Affymetrix chip experiments were performed with Arabidopsis thaliana seedlings treated with one of these two compounds. Experiment Overall Design: Arabidopsis thaliana plants (accession Col-0) were grown on soil in a semi-sterile growth chamber under fluorescent lights (14 hours light, 10 hours dark, 21 centi grades, 100 Einstein/m2/s). Wild type Col-0 plants and the npr1-3 mutant were used in this study. Aerial tissues of 2 week old soil grown Arabidopsis seedlings were sprayed with 100uM 3,5-Dichloroanthranilic acid (DCA), or 100uM 2,6-Dichloroisonicotinic acid (INA) or mock solution and harvested 48h or 6 days after the treatment. Final DMSO concentrations never exceeded 0.002%. Mock treatments were application of 0.002% DMSO in water. For harvesting the aerial plant parts were shock-frozen in liquid nitrogen. Total RNA was isolated from seedlings using TRIZOL (Invitrogen) follwing the manufacturerâ??s instructions. RNA was processed and hybridized to Affymetrix Arabidopsis ATH1 genome array GeneChip following manufacturerâ??s instructions (Affymetrix) by the University of California, Riverside Core Instrument Facility. Three independent biological replicates were performed for each treatment.

2009-03-28 | E-GEOD-13833 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Transcriptome changes triggered by the synthetic defense elicitors DPMP in Arabidopsis thaliana

| PRJNA303108 | ENA

Transcriptome changes triggered by the synthetic defense elicitors DPMP in Arabidopsis thaliana

Project description:DPMP (2,4-dichloro-6-{(E)-[(3-methoxyphenyl)imino]methyl}phenol ) is a newly identified synthetic defense elicitor. In order to uncover transcriptional patterns associated with defense activation, we profiled by mRNA-seq responses triggered in 14 d-old plate-grown Arabidopsis seedlings by continuous exposure to 3 µM DPMP or Mock.

2016-11-22 | GSE75288 | GEO

Expression analysis of Arabidopsis thaliana Col-0 and parg1 with and without PARP inhibitor (3AB, 3MB), with and without MAMP (flg22, elf18)

Project description:Genome-wide gene expression analysis of the effects of PARP inhibitor (3AB) and, separately, a parg1 knockout, on early microbe-associate molecular pattern (MAMP)-induced gene expression in the plant basal defense response. Arabidopsis thaliana wild-type (Col-0), 3AB-treated and parg1-2 T-DNA knockout plants responding to the MAMP elicitors flg22 or elf18 were studied. The mutant and PARP inhibitors analyzed in this study are further described in Adams-Phillips, L.,C., Briggs, A.,G., Bent, A., F. 2010. Disruption of poly(ADP-ribosyl)ation mechanisms alters responses of Arabidopsis to biotic stress. Planty Physiol. 152(1):267-80 DOI: 10.1104/pp.109.148049.

2017-06-20 | GSE100205 | GEO

Comparative transcriptome analysis of TCV-infected wildtype and dcl1-9 mutant Arabidopsis thaliana

Project description:Belonging to the Carmovirus family, Turnip crinkle virus (TCV) is a positive-strand RNA virus that can infect Arabidopsis. Most Arabidopsis ecotypes are highly susceptible to TCV, except for the TCV resistant line Di-17 derived from ecotype Dijon. Previous studies showed that many of the stress related genes have changed significantly after TCV infection. Besides the virus-triggered genes, small RNAs also play critical roles in plant defense by triggering either transcriptional and/or post-transcriptional gene silencing. In this study, TCV-infected wildtype Arabidopsis thaliana and dcl1-9 mutant plants were subjected to transcriptome and small RNA analysis to investigate the role of DCL1 in virus defense network.

2017-01-11 | GSE85067 | GEO

Comparative sRNA-Seq analysis of TCV-infected wildtype and dcl1-9 mutant Arabidopsis thaliana

2017-01-11 | GSE85069 | GEO

Expression data from leafminer-damaged Arabidopsis thaliana

Project description:Plants have developed a complicated resistance system, and they exhibit various defense patterns in response to different attackers. However, the determine factors of plant defense patterns are still not clear. Here, we hypothesized that damage patterns of plant attackers play an important role in determining the plant defense patterns. To test this hypothesis, we selected leafminer, which has a special feeding pattern more similar to pathogen damage than chewing insects, as our model insect, and Arabidopsis thaliana as the response plants. The local and systemic responses of Arabidopsis thaliana to leafminer feeding were investigated using the Affymetrix ATH1 genome array. Damaged leaves of Arabidopsis thaliana for local damage analysis and the intact leaves on the same plant for systemic damage analysis were separately frozen by liquid nitrogen. Then, we used an Affymetrix ATH1 Arabidopsis microarray to study the expression changes pattern of Arabidopsis thaliana to pea leafminers damage, both locally (LI) and systemically (SI). We downloaded data from the web database and used hierarchical clustering to explore the relationships of Arabidopsis thaliana expression pattern to different kinds of attackers.

2014-05-26 | E-GEOD-38281 | biostudies-arrayexpress

Arabidopsis thaliana cultivar:Columbia-0

Project description:Dose-dependency of BHTC-triggered transcriptome changes in Arabidopsis thaliana

| PRJNA297270 | ENA

Flg22 treatment of Brassicaceae and Arabidopsis thaliana accessions

Project description:We used RNA-seq to profile gene expression changes during flg22 activated pattern-triggered immunity in multiple Brassicaceae including Capsella rubella, Cardamine hirsuta and Eutrema salsugineum as well as in multiple Arabidopsis thaliana accessions. This allows comparative transcriptomics within and across species to investigate the evolution of stress-responsive transcrption changes in these species.

2021-03-11 | GSE115991 | GEO

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