Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Arabidopsis thaliana seedlings treated with synthetic defense elicitors 3,5-Dichloroanthranilic acid (DCA) and 2,6-Dichloroisonicotinic acid (INA)


ABSTRACT: DCA (3,5-Dichloroanthranilic acid) is a newly identified synthetic defense elicitor. To perform a comparative analysis of defense responses triggered by DCA and the structurally related defense inducer INA (2,6-Dichloroisonicotinic acid) Affymetrix chip experiments were performed with Arabidopsis thaliana seedlings treated with one of these two compounds. Experiment Overall Design: Arabidopsis thaliana plants (accession Col-0) were grown on soil in a semi-sterile growth chamber under fluorescent lights (14 hours light, 10 hours dark, 21 centi grades, 100 Einstein/m2/s). Wild type Col-0 plants and the npr1-3 mutant were used in this study. Aerial tissues of 2 week old soil grown Arabidopsis seedlings were sprayed with 100uM 3,5-Dichloroanthranilic acid (DCA), or 100uM 2,6-Dichloroisonicotinic acid (INA) or mock solution and harvested 48h or 6 days after the treatment. Final DMSO concentrations never exceeded 0.002%. Mock treatments were application of 0.002% DMSO in water. For harvesting the aerial plant parts were shock-frozen in liquid nitrogen. Total RNA was isolated from seedlings using TRIZOL (Invitrogen) follwing the manufacturerâ??s instructions. RNA was processed and hybridized to Affymetrix Arabidopsis ATH1 genome array GeneChip following manufacturerâ??s instructions (Affymetrix) by the University of California, Riverside Core Instrument Facility. Three independent biological replicates were performed for each treatment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Thomas Eulgem 

PROVIDER: E-GEOD-13833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

The synthetic elicitor 3,5-dichloroanthranilic acid induces NPR1-dependent and NPR1-independent mechanisms of disease resistance in Arabidopsis.

Knoth Colleen C   Salus Melinda S MS   Girke Thomas T   Eulgem Thomas T  

Plant physiology 20090320 1


Immune responses of Arabidopsis (Arabidopsis thaliana) are at least partially mediated by coordinated transcriptional up-regulation of plant defense genes, such as the Late/sustained Up-regulation in Response to Hyaloperonospora parasitica (LURP) cluster. We found a defined region in the promoter of the LURP member CaBP22 to be important for this response. Using a CaBP22 promoter-reporter fusion, we have established a robust and specific high-throughput screening system for synthetic defense eli  ...[more]

Similar Datasets

2009-03-20 | GSE13833 | GEO
2023-12-25 | GSE211047 | GEO
2013-11-14 | E-GEOD-44793 | biostudies-arrayexpress
2010-08-09 | E-GEOD-18329 | biostudies-arrayexpress
2010-11-21 | E-GEOD-23617 | biostudies-arrayexpress
2007-02-08 | GSE4203 | GEO
2014-11-03 | E-GEOD-57449 | biostudies-arrayexpress
2020-01-20 | PXD014698 | Pride
2020-01-20 | PXD014699 | Pride
2014-06-20 | E-GEOD-51720 | biostudies-arrayexpress