Project description:Newly generated plasma cells in secondary lymphoid organs migrate to niches in the bone marrow, wherein they survive as long-lived plasma cells (LLPCs). Although characterization of LLPCs has been well performed, it is still unclear what is key determinant(s) for longevity of plasma cells. One postulated model is that heterogeneity of plasma cells is formed by such as BCR affinity and/or microenvironment at the induction site, and some of these variables may instruct their longevity. Here, we found that, among newly generated IgG plasma cells, integrin b7hi marks plasma cells prone to home to the bone marrow, whereas integrin b7lo cells remain in secondary lymphoid organs.
Project description:We found that, among newly generated IgG plasma cells, integrin b7hi marks plasma cells prone to home to the bone marrow, whereas integrin b7lo cells remain in secondary lymphoid organs. Moreover, we also found that the differences in KLF2 expression between b7hi and b7lo populations in secondary lymphoid organs is likely to explain that only integrin b7hi plasma cells can egress. Next, to look for the functional target(s) downstream of KLF2 involved in egress of newly generated plasma cells, we carried out RNA-seq analysis of KLF2-sufficient and -deficient splenic plasma cells. Wildtype control splenic plasma cells were further divided into integrin b7hi and b7lo populations. We identified S1pr1 and Itgam as functional targets downstream of KLF2.
Project description:This trancriptome analysis reveals the first detailed analysis on how the remnant lens epithelial cells (LECs) greatly reprogram their transcriptome by 24 hours post cataract surgery (PCS) and the dependence of this phenomenom on endogenous β8 integrin gene expression.
Project description:We performed single cell RNA sequencing to analyze the transcriptional profile of gliogenic NS/PCs and neurogenic NS/PCs derived from the same parental feeder-free iPSCs.
Project description:To elucidate the molecular basis underlying early female reproductive development, Here, we used high-throughput tag-sequencing analysis to identify genes preferentially expressed in female meiocytes (FMs) by comparing gene expression profiles from wild type ovules undergoing megasporogenesis with those from the lacking megasporogenesis spl mutant ovules. A total of 862 genes were identified as FMs with levels that are consistently reduced in spl ovules in two biological replicates. Genes involved in biogenesis, metabolism, transporter activity and DNA binding were over-represented in female meiocytes, suggesting that female meiocytes are synthetically and metabolically more active. Total mRNA profiles of stage 10-11 old M-oM-,M-^Boral buds wild type (WT) and SPL-/- ovules with placenta were generated by deep sequencing, Repeat twice, using Illumina adaptor 2 (GEX adapter 2).
Project description:Purpose: Genome-wide DNA-binding analysis for E(spl)m8 in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on E(spl)m8 driven by Dl-Gal4 Results: