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Ldb1 establishes multi-enhancer networks to regulate gene expression

ABSTRACT: Ldb1 establishes multi-enhancer networks to regulate gene expression

PROVIDER: PRJNA1107208 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28878060_1.fastq.gz	Fastqsanger.gz
	SRR28878060_2.fastq.gz	Fastqsanger.gz
	SRR28878061_1.fastq.gz	Fastqsanger.gz
	SRR28878061_2.fastq.gz	Fastqsanger.gz
	SRR28878062_1.fastq.gz	Fastqsanger.gz

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Ldb1 establishes multi-enhancer networks to regulate gene expression

Project description:Ldb1 establishes multi-enhancer networks to regulate gene expression

| PRJNA1070275 | ENA

Ldb1 establishes multi-enhancer networks to regulate gene expression

Project description:How enhancers specifically connect to gene promoters is still unclear. Besides the CTCF/cohesin machinery, only a small set of nuclear factors have been studied for a direct role in physically connecting regulatory elements. Here, we show via acute degradation experiments that LDB1 directly and broadly promotes enhancer-promoter loops. Utilizing multiple degron systems, we demonstrate that most endogenous LDB1-mediated contacts can form in the absence of CTCF, cohesin and YY1. Furthermore, an engineered/forced, LDB1-driven chromatin loop can form in the absence of cohesin. Yet, in a fraction of cases cohesin driven extrusion may promote LDB1 anchored loops. Leveraging the dynamic reorganization of nuclear architecture during the transition from mitosis to G1 phase, we establish a relationship between LDB1-dependent interactions in the context of TAD organization and gene activation. Tri-C and Region Capture Micro-C reveal that LDB1 organizes multi-enhancer networks to activate transcription. This establishes LDB1 as direct driver of connectivity between regulatory elements.

2024-11-27 | GSE266450 | GEO