AMH protects the ovary from doxorubicin by regulating cell fate and the response to DNA damage
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1107821 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28897200_1.fastq.gz | Fastqsanger.gz | |||
| SRR28897200_2.fastq.gz | Fastqsanger.gz | |||
| SRR28897201_1.fastq.gz | Fastqsanger.gz | |||
| SRR28897201_2.fastq.gz | Fastqsanger.gz | |||
| SRR28897202_1.fastq.gz | Fastqsanger.gz |
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Project description:we aim to explore the single-cell mechanisms behind ovarian damage in prepubertal mice following doxorubicin (DOX) treatment, and how Anti-Müllerian hormone (AMH) prevents chemotherapy-induced pathological damage, using single-cell RNA sequencing as well as quantitative and morphometric histological evaluation.
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Project description:Anti-müllerian hormone (AMH) has an inhibitory effect on ovarian follicle development. However, the mechanism by which AMH regulates folliculogenesis remains to be elucidated. In this study we aimed to investigate the changes in transcriptome of preantral to small antral mice follicles after culturing with AMH and thereby identify candidate genes to be involved. This study is a repetition of a previous study (GEO accession .no.: GSE56737) at the concentration of 50 ng/mL. The overall design are almost similar to GSE56737 except that it was performed in quadruplicate.
2015-05-01 | GSE63907 | GEO
Project description:The current study was designed to investigate the actions of Anti-MM-CM-<llerian Hormone (AMH) on primordial follicle assembly. Ovarian primordial follicles develop from the breakdown of oocyte nests during fetal development for the human and immediately after birth in rodents. AMH was found to inhibit primordial follicle assembly, decrease the initial primordial follicle pool size and promote the persistence of small oocyte nests in a rat ovarian organ culture. The AMH expression was found to be primarily in the stromal tissue of the ovaries at this period of development, suggesting a stromal-epithelial cell interaction for primordial follicle assembly. AMH was found to promote alterations in the ovarian transcriptome during primordial follicle assembly with over 200 genes with altered expression. A gene network was identified suggesting a potential central role for the Fgf2/Nudt6 antisense transcript in the follicle assembly process. A number of signal transduction pathways are regulated by AMH actions on the ovarian transcriptome, in particular the transforming growth factor M-bM-^@M-^S beta (TGFM-CM-^_) signaling process. AMH is the first hormone/protein shown to have an inhibitory action on primordial follicle assembly. Due to the critical role of the primordial follicle pool size for female reproduction, elucidation of the factors, such as AMH, that regulate the assembly process will provide insights into potential therapeutics to manipulate the pool size and female reproduction. We used microarrays to determine genes expressed differentially between control and AMH (Anti-MM-CM-<llerian Hormone) treated P0 ovary RNA samples from 3 control groups are compared to 3 AMH treated ovary groups
2011-03-04 | E-GEOD-25629 | biostudies-arrayexpress
Project description:Anti-müllerian hormone (AMH) has an inhibitory effect on ovarian follicle development. However, the mechanism by which AMH regulates folliculogenesis remains to be elucidated. In this study we aimed to investigate the changes in transcriptome of preantral to small antral mice follicles after culturing with AMH and thereby identify candidate genes to be involved. This study is a repetition of a previous study (GEO accession .no.: GSE56737) at the concentration of 50 ng/mL. The overall design are almost similar to GSE56737 except that it was performed in quadruplicate. Preantral to small antral follicles were collected from dissected ovaries from six to eight weeks old female C57BL/6Tac mice. Twelve hour as well as 24 hour experiments were performed in one fixed concentration of AMH and an untreated control. The experiments were performed in quadroplicate. In total 16 samples: 2 x conc. (control and 50 ng/ml AMH), 2 x time points, 4 x experiments (quadroplicate). Samples are pairwise comparable within each experiment, that is, sample 1-2 or 3-4 or 5-6 or 7-8 or 9-10 or 11-12 or 13-14 or 15-16. Data are also comparable groupwise; (1,5,9,13) vs (2,6,10,14) or (3,7,11,15) vs (4,8,12,16).
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