Project description:Interventions: case series:none
Primary outcome(s): Blood lipids;Colorectal polyps, colorectal cancer occurrence and development
Study Design: Sequential
Project description:Human monocytes are phagocytic leucocytes which circulate in the peripheral blood and play important roles in immunity and inflammation. They also represent circulating M-^QmetabolicM-^R sentinels in plasma, uptake lipids following food intake, and contribute to atherosclerotic plaque formation. We therefore investigated their homeostatic responses to food intake in vivo and to determine the response of human monocytes to food intake. We isolated monocyte subsets from the blood of healthy volunteers one hour before and four hours after a western type lunch.
Project description:In this epigenome-wide association study (EWAS), we examined the associations between PCDD, PCDF, and PCB exposures and DNA methylation. Whole blood DNA methylation was measured using Illumina EPIC arrays (n=292). We modeled lipid-adjusted toxic equivalencies (TEQs) for: ΣDioxins (sum of 28 PCDDs, PCDFs, cPCBs, and mPCBs), PCDDs, PCDFs, cPCBs, and mPCBs using robust multivariable linear regression adjusting for age, race, sex, smoking, total lipids, and estimated percentages of six blood cell types.
Project description:We conducted a lipidomic analysis of the whole body of female Aedes aegypti mosquitoes 14 at different time points over the course of feeding and reproduction. There were temporal biphasic 15 increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h post 16 blood meal. During these two increases, the abundance of many lipids dropped while body weight 17 remained stable, probably reflecting blood lipid digestion and the synthesis of vitellogenin in this 18 period. A concerted temporal pattern was particularly strong at the second peak for membrane and 19 signalling lipids such as PE, PI, CL, HexCer and LPA. Lyso-glycerophospholipids showed three 20 distinct change patterns which are functionally related: LPE and LPC, which are membrane lipids, 21 showed little change; LPA, a signalling lipid, showed a dramatic increase from 16 to 30 h PBM; LPI, 22 a bioactive lipid, and both LPG and LPS which are bacterial membrane lipids, showed one signifi-23 cant increase from the time of feeding to 16 hours post blood meal. The result of our study on the 24 anautogenous insect Ae. aegypti point to specific lipids likely to be important in the reproductive 25 process with a role in the formation and growth of ovarian follicles.
Project description:Raw files corresponding to the epilipidomic dataset for identification and relative quantification of oxidized complex lipids in the blood plasma of lean and obese individuals.
Project description:The purpose of the study is to investigate phospholipid ligands and their receptors in inflammatory bowel diseases and colon cancer. Several new species of lipids have been recently discovered which are able to transmit information to cancer cells in the large intestine. The lipids and their responsive receptors build an axis that is thought to influence the development of inflammation and cancer.
Project description:Lipids play a critical role in the skin as components of the epidermal barrier and as sig-naling molecules. Atopic dermatitis in dogs is associated with changes in the lipid composition of the skin, but whether these precede the onset of dermatitis or occur secondary to the dermatitis is unclear. We applied rapid lipid profiling mass spectrometry methods to skin and blood samples of dogs and determined changes following systemic treatment. Thirty control dogs and 30 atopic dogs with mild to moderate dermatitis were enrolled. Marked differences in lipid profiles were observed between control, nonlesional and lesional skin of dogs. Additionally, there were significant altera-tions in the lipid composition of the blood samples indicating systemic changes in lipid metabolism. Treatment with oclacitinib or lokivetmab resulted in a significant decrease of the disease clinical severity associated with changes in skin and blood lipids. A set of lipid features of the skin were selected as biomarkers that classified samples as control or atopic dermatitis with 95% accuracy, whereas blood lipids discriminated between control and atopic dogs with 82% accuracy. These data suggest that atopic dermatitis is a systemic disease and support the use of rapid lipid profiling to identify novel biomarkers.
Project description:The avascular nature of cartilage makes it a unique tissue in the human body, but whether and how the absence of blood vessels regulates chondrogenesis remains unknown. Obstruction of vascular invasion during bone healing favours chondrogenic over osteogenic differentiation of skeletal progenitor cells, a process we observed to be driven by a decreased availability of extracellular lipids. To understand how lipid deprivation activates the chondrogenic programme, we profiled mRNA expression of C3H10T1/2 skeletal progenitors exposed to lipid deprivation for 1, 3 or 6 hours.