Project description:Interventions: case series:none
Primary outcome(s): Blood lipids;Colorectal polyps, colorectal cancer occurrence and development
Study Design: Sequential
Project description:Human monocytes are phagocytic leucocytes which circulate in the peripheral blood and play important roles in immunity and inflammation. They also represent circulating M-^QmetabolicM-^R sentinels in plasma, uptake lipids following food intake, and contribute to atherosclerotic plaque formation. We therefore investigated their homeostatic responses to food intake in vivo and to determine the response of human monocytes to food intake. We isolated monocyte subsets from the blood of healthy volunteers one hour before and four hours after a western type lunch.
Project description:We conducted a lipidomic analysis of the whole body of female Aedes aegypti mosquitoes 14 at different time points over the course of feeding and reproduction. There were temporal biphasic 15 increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h post 16 blood meal. During these two increases, the abundance of many lipids dropped while body weight 17 remained stable, probably reflecting blood lipid digestion and the synthesis of vitellogenin in this 18 period. A concerted temporal pattern was particularly strong at the second peak for membrane and 19 signalling lipids such as PE, PI, CL, HexCer and LPA. Lyso-glycerophospholipids showed three 20 distinct change patterns which are functionally related: LPE and LPC, which are membrane lipids, 21 showed little change; LPA, a signalling lipid, showed a dramatic increase from 16 to 30 h PBM; LPI, 22 a bioactive lipid, and both LPG and LPS which are bacterial membrane lipids, showed one signifi-23 cant increase from the time of feeding to 16 hours post blood meal. The result of our study on the 24 anautogenous insect Ae. aegypti point to specific lipids likely to be important in the reproductive 25 process with a role in the formation and growth of ovarian follicles.
Project description:In this epigenome-wide association study (EWAS), we examined the associations between PCDD, PCDF, and PCB exposures and DNA methylation. Whole blood DNA methylation was measured using Illumina EPIC arrays (n=292). We modeled lipid-adjusted toxic equivalencies (TEQs) for: ΣDioxins (sum of 28 PCDDs, PCDFs, cPCBs, and mPCBs), PCDDs, PCDFs, cPCBs, and mPCBs using robust multivariable linear regression adjusting for age, race, sex, smoking, total lipids, and estimated percentages of six blood cell types.
Project description:Raw files corresponding to the epilipidomic dataset for identification and relative quantification of oxidized complex lipids in the blood plasma of lean and obese individuals.