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Lipidomic profiling reveals concerted temporal patterns of 2 functionally related lipids in Aedes aegypti females following 3 blood feeding

ABSTRACT: We conducted a lipidomic analysis of the whole body of female Aedes aegypti mosquitoes 14 at different time points over the course of feeding and reproduction. There were temporal biphasic 15 increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h post 16 blood meal. During these two increases, the abundance of many lipids dropped while body weight 17 remained stable, probably reflecting blood lipid digestion and the synthesis of vitellogenin in this 18 period. A concerted temporal pattern was particularly strong at the second peak for membrane and 19 signalling lipids such as PE, PI, CL, HexCer and LPA. Lyso-glycerophospholipids showed three 20 distinct change patterns which are functionally related: LPE and LPC, which are membrane lipids, 21 showed little change; LPA, a signalling lipid, showed a dramatic increase from 16 to 30 h PBM; LPI, 22 a bioactive lipid, and both LPG and LPS which are bacterial membrane lipids, showed one signifi-23 cant increase from the time of feeding to 16 hours post blood meal. The result of our study on the 24 anautogenous insect Ae. aegypti point to specific lipids likely to be important in the reproductive 25 process with a role in the formation and growth of ovarian follicles.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Aedes Aegypti (ncbitaxon:7159)

SUBMITTER: Ary A. Hoffmann

PROVIDER: MSV000091430 | MassIVE | Tue Mar 07 16:16:00 GMT 2023

REPOSITORIES: MassIVE

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GNPS - Lipidomic profiling reveals concerted temporal patterns of 2 functionally related lipids in Aedes aegypti females following 3 blood feeding

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