Project description:We performed a laboratory experiment with vegetative gametophytes of the kelp Saccharina latissima and exposed the gametophytes to three temperatures (4°C, 12°C and 20°C) by sex (female, male) for 14 days.
Project description:Effect of PAR and temperature stress on the gene expression Saccharina latissima. Total RNA of stress treatments (low PAR 2° and 17°C, high PAR 2° and 17°C) was hybridized against the control treatment (low PAR 12°C); hybridizations were carried out in 4 replicates.
Project description:To explore possible interactive effects of UV-radiation, temperature and growth conditions, cultivated and field sporophytes of Saccharina latissima were exposed for 24h to UV-radiation at three different temperatures (2,7 & 12°C). Gene expression profiles under UV-radiation at different temperatures were assessed through microarray hybridizations, afterwards comparisons of gene expression profiles in field and culture sporophytes were carried out.
Project description:Sporophytes of Saccharina latissima were exposed for two weeks to 12 different combinations of photosynthetically active radiation and UVR at three different temperatures (2,7,12 C). Maximum quantum yield of photosystem II was determined twice a week during the experimental duration for observing the extent of photoinhibition. For investigating molecular mechanisms of acclimation to high photosynthetically radiation, and UVR gene expression profiles were assessed through microarray hybridizations.
