Project description:In order to identify the direct target of TFEB, we performed a ChIP-seq in HEK293-PFL-TFEB cells where, after removal of tetracycline, samples were collected in duplicates at 18, 36 and 90 hours
2020-09-28 | GSE98246 | GEO
Project description:Hydrocarbon removal by the bioaugmentation of biosurfagctant-producing bacteria
Project description:The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. Upon tetracycline removal but inthe presence of LIF (Tet OFF condition) Stat3F is expressed and the cells differentiate despite the presence of LIF. We thus performed microarray analysis of the transcriptome of ES cells after 16h, 24h and 48h after Tetracycline removal (OFF16; OFF24 and OFF48) respectively)and compared them to Gs2 ES cells maintained in the continuous presence of LIF and tetracyclin (LIF/ON). Keywords: time-course
2004-07-01 | GSE1151 | GEO
Project description:Metagenomic analysis of tetracycline degradation consortia
Project description:The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. Upon tetracycline removal but inthe presence of LIF (Tet OFF condition) Stat3F is expressed and the cells differentiate despite the presence of LIF. We thus performed microarray analysis of the transcriptome of ES cells after 16h, 24h and 48h after Tetracycline removal (OFF16; OFF24 and OFF48) respectively)and compared them to Gs2 ES cells maintained in the continuous presence of LIF and tetracyclin (LIF/ON).
Project description:Oxygen-stable HIF1 was inducibly expressed in cardiomyocytes of double transgenic mice. Specifically, tetracycline-inducible HIF-PPN mice (Pro402, Pro564 and Asn803 of HIF1a were mutated to Ala residues to limit degradation, termed HIF-PPN) were crossed to a-MHC-tTA expressing mice. HIF-PPN expression was induced by removal of doxycycline for 3 days and compared to control hearts that remained on doxycycline (uninduced).
2019-01-09 | GSE119535 | GEO
Project description:16S rRNA gene Illumina sequences from bioaugmentation in Mn removal filters
Project description:The aim of this experiment was to determine if the development of resistance to antibiotics can be driven by the concentration and speciation of Cu. Experimental setup was designed to investigate two hypotheses for which two strains of Gram- bacteria have been selected: - Do TE enhance AR in resistant bacteria? Resistant strain: Bioluminescent Pseudomonas aeruginosa PAO1 (Xen41, Tetracycline resistant) - Do TE induce AR in sensitive bacteria? Sensitive strain: Pseudomonas aeruginosa PAO1 (Wild Type)
Project description:PU.1 is an Ets family transcription factor that is essential for the differentiation of both myeloid and lymphoid cells. PU.1 is down-regulated in classical Hodgkin lymphoma cells via methylation of the PU.1 promoter. To evaluate whether down-regulation of PU.1 is essential for the growth of cHL cells, we generated L428 derived cell lines conditionally express PU.1 by tet-off system (designated L428tetPU.1). Conditonally expressed PU.1 by tetracycline removal induced complete growth arrest and apoptosis in L428 cells. To elucidate the mechanisms underlying cell cycle arrest and apoptosis induced by PU.1, we compared gene expression profiles of L428tetPU.1 cells 0, 1 and 3 days after PU.1 induction, by DNA microarray. We extracted total RNA from L428tetPU.1 cells 0, 1 and 3 days after PU.1 induction by tetracycline removal. We compared gene expression profiles of KL428tetPU.1 cells 0, 1 and 3 days after PU.1 induction using DNA microarray analysis. 4 independent experiments were performed with each RNA samples.