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Root nodule metagenome

ABSTRACT: Metatranscriptome of root nodules from Medicago truncatula inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_truncatula_C

PROVIDER: PRJNA1109878 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29155532_1.fastq.gz	Fastqsanger.gz
	SRR29155532_2.fastq.gz	Fastqsanger.gz

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root nodule metagenome

Project description:Metatranscriptome of root nodules from Medicago truncatula inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_truncatula_A

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Nodule Zone Specific Proteome - Integrated analysis of zone-specific protein and metabolite profiles within nitrogen-fixing Medicago truncatula-Sinorhizobium medicae nodules

Project description:Root nodules of the medicago truncatula-sinorhizobium meliloti plant-bacterail symbiotic model system were hand sectioned followed by LCMS. Sections were made to separate different developmental zones along the nodules longitudinal axis.

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Expression of Nodule Cysteine-Rich (NCR) Defensin-Like (DEFL) genes among four different accessions of Medicago truncatula inoculated with two different rhizobial strains.

Project description:We have undertaken a detailed study to identify expression polymorphism of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in four different accessions of Medicago truncatula. Each accession was inoculated with two different rhizobial strains to assess the NCR expression differences caused by host-symbiont specificity. The NCRs displayed significant expression differences among the ecotypes but had subtle differences in gene expression because of strain difference. We used a custom Affymetrix chip containing 684 probe sequences of Medicago DEFLs to explore the expression polymorphism of NCRs in nodules inoculated with Sinorhizobium meliloti 1021(Sm1021) and Sinorhizobium medicae (SmA321) totalling 8 different treatments. Each treatment was supported by three biological replicates giving a grand total of 24 samples.

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