ABSTRACT: Metatranscriptome of root nodules from Medicago truncatula inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_truncatula_B
Project description:Metatranscriptome of root nodules from Medicago truncatula inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_truncatula_A
Project description:Metatranscriptome of root nodules from Medicago truncatula inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_truncatula_C
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_S_A
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_S_B
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_L_C
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_S_C
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_L_A
Project description:Metatranscriptome of root nodules from Medicago sativa inoculated with Sinorhizobium meliloti 1021 RmP4566 from North Dakota State University, Fargo, ND, USA - RmP4566_M_sativa_L_B
Project description:Root nodules of the medicago truncatula-sinorhizobium meliloti plant-bacterail symbiotic model system were hand sectioned followed by LCMS. Sections were made to separate different developmental zones along the nodules longitudinal axis.
Project description:We have undertaken a detailed study to identify expression polymorphism of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in four different accessions of Medicago truncatula. Each accession was inoculated with two different rhizobial strains to assess the NCR expression differences caused by host-symbiont specificity. The NCRs displayed significant expression differences among the ecotypes but had subtle differences in gene expression because of strain difference. We used a custom Affymetrix chip containing 684 probe sequences of Medicago DEFLs to explore the expression polymorphism of NCRs in nodules inoculated with Sinorhizobium meliloti 1021(Sm1021) and Sinorhizobium medicae (SmA321) totalling 8 different treatments. Each treatment was supported by three biological replicates giving a grand total of 24 samples.