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The effect of knockout Brd7 on 4T07-TGL cells under in vitro condition [RNA-seq]

ABSTRACT: The effect of knockout Brd7 on 4T07-TGL cells under in vitro condition [RNA-seq]

PROVIDER: PRJNA1110227 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28994800_1.fastq.gz	Fastqsanger.gz
	SRR28994800_2.fastq.gz	Fastqsanger.gz
	SRR28994801_1.fastq.gz	Fastqsanger.gz
	SRR28994801_2.fastq.gz	Fastqsanger.gz
	SRR28994802_1.fastq.gz	Fastqsanger.gz

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The effect of knockout Brd7 on 4T07-TGL cells under in vitro condition

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Project description:Metastasis is a major cause of mortality in cancer and is likely influenced by epigenetic and gene regulatory factors. Using an in vivo screen, we demonstrated that several subunits of the polybromo-associated BAF (PBAF) complex, particularly BRD7, were required for breast cancer metastatic dormancy in lung. BRD7 loss induced metastatic outgrowth, along with modifications in epigenomic landscapes and upregulated oncogenic signaling. Breast cancer cells harboring BRD7 loss also reprogrammed the surrounding immune microenvironment, downregulating MHC-1 expression and promoting a pro-metastatic cytokine profile. Flow cytometric and single-cell analyses revealed increased levels of pro-tumorigenic neutrophils, CD8+ exhausted T cells, and CD4+ stress response T cells in lungs harboring BRD7-deficient metastases. Finally, attenuating this immunosuppressive milieu by neutrophil depletion, neutrophil extracellular trap (NET) inhibition, or immune checkpoint therapy abrogated metastatic outgrowth. These findings, for the first time, implicate BRD7 and PBAF in metastasis, pointing to targetable underlying mechanisms involving specific immune cell compartments.

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Project description:Transcriptional profiling of human BJ fibroblasts comparing control FF shRNA expressing cells vs. BRD7 shRNA expressing cells under two conditions, either untreated or treated with 8uM nutln-3a for 8 hours. This experiment was done using two independent shRNAs targeting BRD7. Nutlin-3a was used to stabilize p53 and induce its transcriptional activity. Two-condition experiment, FF shRNA cells vs. BRD7 shRNAs cells in two experimental conditions, either untreated or treated with nutlin-3a.

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