Project description:We reported the first study of transcriptome analyses using the Illumina HiSeq4000 platform for low temperature stress on callus of Panax ginseng. In total, over 546,139,358 clean reads were produced and 44,953 unigenes were assembled; Q30 results are above 95%. Differentially Expressed Gene analysis identified 9,435 and 6,345 unigenes which were expressed either higher or lower in treatment group samples(T) when compared with control group (C). We can find that the differential gene is more expressed in the T_4 vs C_4 group than in the other comparison groups, thus demonstrating that the differential gene expression is more active. Chilling treatment for 2th-6th day, the total saponin content of the treatment group was significantly higher than that of the control group, and reached the maximum on the forth day of treatment, and then gradually decreased. There is a synergistic trend between differential gene expression and saponin content at different treatment times. The accuracy of these de novo sequences was validated by RT-qPCR analysis. A number of transcription factor genes were also filtrated that presumably related to environmental cold stress. These data will enrich our knowledge of triterpenoid saponin biosynthesis and provide a theoretical foundation for molecular research on Panax ginseng.
Project description:In order to research the ginseng leaf-stem gene expression profiles of and dig out its function genes in the leaf-expansion period, the transcriptomic sequencing technology was set up the first time for five years the transcription of the Panax ginseng leaf-stem in the leaf-expansion period.
