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Single cell sequencing of stromal vascular fraction from facial infiltrating lipomatosis and normal facial adipose tissue

ABSTRACT: Single cell sequencing of stromal vascular fraction from facial infiltrating lipomatosis and normal facial adipose tissue

PROVIDER: PRJNA1112852 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29070762_1.fastq.gz	Fastqsanger.gz
	SRR29070762_2.fastq.gz	Fastqsanger.gz
	SRR29070763_1.fastq.gz	Fastqsanger.gz
	SRR29070763_2.fastq.gz	Fastqsanger.gz
	SRR29070764_1.fastq.gz	Fastqsanger.gz

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Single cell sequencing of stromal vascular fraction from facial infiltrating lipomatosis and normal facial adipose tissue

Project description:Facial infiltrating lipomatosis is characterized by excessive growth of adipose tissue, The etiology is associated with somatic PIK3CA variant, but the specific mechanisms are not yet fully understood. In this study, we collected facial adipose tissue from both FIL patients and non-FIL individuals, isolated the stromal vascular fraction (SVF) and performed single-cell transcriptome sequencing on these samples. We mapped out the cellular landscape within the SVF and specifically focused on a deeper analysis of fibro-adipogenic precursor cells (FAPs).

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Project description:Microarray studies were performed to identify expression patterns by cryopreserved adipose stromal vascular fraction (SVF) preparations that were prepared by three different veterinary stem cell companies from the same source of canine adipose tissue. [Samples 1 - 3] Three equal samples of approximately 20 grams of adipose tissue were shipped on ice to 3 different companies which specialize in adipose-derived veterinary stem cell therapy. All 3 SVF preparations were cryopreserved at their respective facilities. Approximately one month later, cryopreserved samples were retrieved from all companies and transported frozen to the University of Kentucky for gene profiling and viability analysis. [Samples 4 - 11] Groups of cells (samples 5, 7, 9, 11) were treated by the stem cell company with photo-activated platelet-rich plasma (PRP) according to their proprietary procedures in order to enhance regenerative properties of the adipose stem cells. These PRP-treated cells were incubated in the investigator's lab for 6 hours at 37 degrees Celsius to allow for expression of genes that were induced by PRP treatment.

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