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Mouse gut metagenome

ABSTRACT: 2023 Nanjing GemPharmatech 60 samples of GMM6 gnotobiotic mice feces and intestinal contents 16SV3V4 amplicon sequencing raw data

PROVIDER: PRJNA1113148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29076600_1.fastq.gz	Fastqsanger.gz
	SRR29076600_2.fastq.gz	Fastqsanger.gz
	SRR29076601_1.fastq.gz	Fastqsanger.gz
	SRR29076601_2.fastq.gz	Fastqsanger.gz
	SRR29076602_1.fastq.gz	Fastqsanger.gz

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Extracellular miRNAs in serum and feces of mice exposed to high-dose radiation

Project description:Exposure to high-dose radiation causes life-threatening serious intestinal damage. Histological analysis is the most accurate method for judging the extent of intestinal damage after death. However, it is difficult to predict the extent of intestinal damage to body samples. Here we focused on extracellular microRNAs (miRNAs) released from cells and investigated miRNA species that increased or decreased in serum and feces using a radiation-induced intestinal injury mouse model. A peak of small RNA of 25–200 nucleotides was detected in mouse serum and feces 72 h after radiation exposure, and miRNA presence in serum and feces was inferred. MiRNAs expressed in the small intestine and were increased by more than 2.0-fold in serum or feces following a 10 Gy radiation exposure were detected by microarray analysis and were 4 in serum and 19 in feces. In this study, miR-375-3p, detected in serum and feces, was identified as the strongest candidate for a high-dose radiation biomarker in serum and/or feces using a radiation-induced intestinal injury model.

2023-12-15 | GSE247876 | GEO

The effect of adipose tissue-specific overexpression of adrenomedullin 2 on the regulation of adipocyte function

Project description:To investigate the effect of adipose tissue-specific overexpression of adrenomedullin 2 on the regulation of adipocyte function, the transgenic mouse line with adipocyte-specific overexpression of the ADM2 gene (aADM2-tg mice) was generated on a C57BL/6J background with the human ADM2 gene driven by the adiponectin (Adipoq) gene promoter (H11-Adipoq-hADM2-Wpre-PolyA mice, Strain NO. T059304). The mice were purchased from GemPharmatech (Nanjing, China). We then performed gene expression profiling analysis using data obtained from RNA-seg of visceral adipose tissue from aADM2-Tg and WT group.

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B.thetaiotaomicron mono-association versus B. thetaiotaomicron co-colonization with B. longum, PR chow, B6

Project description:Genome wide transcriptional comparison of B. thetaiotaomicron mono-association vs. B. thetaiotaomicron bi-association with B. longum. Cecal populations from 10d colonizations with b6 gnotobiotic mice fed a standard-chow polysaccharide rich (PR) diet (n=5 samples/group) were profiled. Total RNA was prepared from cecal contents of 10 day associated gnotobiotic mice and hybridized to B. thetaiotaomicron/B. longum GeneChips.

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Project description:Genome wide transcriptional comparison of B. thetaiotaomicron mono-association vs. B. thetaiotaomicron bi-association with B.animalis or L. casei. Cecal populations from 20d colonizations with B6 gnotobiotic mice fed a standard-chow polysaccharide rich (PR) diet (n=5 samples/group) were profiled. Total RNA was prepared from cecal contents of 20 day associated gnotobiotic mice and hybridized to B. thetaiotaomicron/B. longum GeneChips.

2010-07-01 | E-GEOD-5867 | biostudies-arrayexpress

B.thetaiotaomicron or B. longum mono-association versus B. thetaiotaomicron/B. longum co-colonization, PR chow, NMRI

Project description:Genome wide transcriptional comparison of B. thetaiotaomicron or B. longum mono-association vs. B. thetaiotaomicron bi-association with B. longum. Cecal populations from 10d colonizations with NMRI gnotobiotic mice fed a standard-chow polysaccharide rich (PR) diet (n=5 samples/group) were profiled. Total RNA was prepared from cecal contents of 10 day associated gnotobiotic mice and hybridized to B. thetaiotaomicron/B. longum GeneChips.

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