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Mouse gut metagenome

ABSTRACT: 2024 Nanjing GemPharmatech 14 samples of OMM12 gnotobiotic mice feces 16SV3V4 amplicon sequencing raw data

PROVIDER: PRJNA1113149 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29076821_1.fastq.gz	Fastqsanger.gz
	SRR29076821_2.fastq.gz	Fastqsanger.gz
	SRR29076822_1.fastq.gz	Fastqsanger.gz
	SRR29076822_2.fastq.gz	Fastqsanger.gz
	SRR29076823_1.fastq.gz	Fastqsanger.gz

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Project description:To investigate the effect of adipose tissue-specific overexpression of adrenomedullin 2 on the regulation of adipocyte function, the transgenic mouse line with adipocyte-specific overexpression of the ADM2 gene (aADM2-tg mice) was generated on a C57BL/6J background with the human ADM2 gene driven by the adiponectin (Adipoq) gene promoter (H11-Adipoq-hADM2-Wpre-PolyA mice, Strain NO. T059304). The mice were purchased from GemPharmatech (Nanjing, China). We then performed gene expression profiling analysis using data obtained from RNA-seg of visceral adipose tissue from aADM2-Tg and WT group.

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Project description:Gut microbiome modulates the host immune development, yet the functional contribution of gut fungi remains elusive. We previously showed that mice colonized only with fungi displayed allergic features and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed proteomic analyses of feces and small intestine of gnotobiotic mice colonized with either bacteria, fungi, or both. Comparison of fecal metaproteomic profiles between mouse groups yielded broad changes in the relative levels of bacterial, fungal and mouse proteins. Many of the detected fungal proteins have been previously reported as a part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the small intestine impacted essential cellular pathways, including lipid metabolism and apoptosis. The results show how fungal colonization impacts the host proteome and suggest an influence on the host final cellular phenotype.

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