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ABSTRACT: Study of the splicing in Thumpd2 knockout mice

PROVIDER: PRJNA1114654 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29125083.fastq.gz	Fastqsanger.gz
	SRR29125084.fastq.gz	Fastqsanger.gz
	SRR29125085.fastq.gz	Fastqsanger.gz
	SRR29125086.fastq.gz	Fastqsanger.gz
	SRR29125087.fastq.gz	Fastqsanger.gz

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Muscle Developmental Defects in Heterogeneous Nuclear Ribonucleoprotein A1 Knockout Mice

Project description:Transcriptome analysis of total RNA samples from heart tissue of knockout mice Alternative splicing is the main mechanism to increase protein diversity from an mRNA. Heterogeneous ribonucleoprotein (hnRNP) family members are vital regulators of alternative splicing. The hnRNP A1 is the most well-known protein in this family, but its role in embryonic development is not well understood. We generated hnRNP A1 knockout mice to study the function of hnRNP A1 in vivo. The hnRNP A1-depleted mice showed embryonic lethality because of muscle developmental defects. In a previous study, cellular hnRNP A2/B1 was reported to be capable of compensating for the expression of hnRNP A1. However, this phenomenon did not occur in the hnRNP A1 heterozygous mice in vivo. We demonstrated that hnRNP A1 regulated muscle-related genes expression and alternative splicing. In summary, our data demonstrated that hnRNP A1 plays a critical role in embryonic muscle development. Understanding the effects of hnRNP A1 in vivo may help to define the function of hnRNP A1 in alternative splicing.

2016-12-05 | GSE79076 | GEO

Ablation of the epithelial-specific splicing factor Esrp1 results in ureteric branching defects and reduced nephron number

Project description:Evaluation of splicing in ureteric epithelium in Esrp1;Esrp2 double knockout mice compared to Esrp1+/+;Esrp2-/- control mice.

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Knockout mice reveal an essential role for Epithelial splicing regulatory proteins (Esrps) in mammalian development and epithelial splicing in vivo

Project description:Tissue- and cell-type specific regulators of alternative splicing (AS) are an essential layer of posttranscriptional gene regulation necessary for normal cellular function, patterning, and development. Here we report the Epithelial splicing regulatory proteins (Esrps) are required for patterning of multiple organs, with loss of both paralogs, Esrp1 and Esrp2, resulting in increasingly severe phenotypes. Global profiling of the Esrp splicing regulatory network from total epidermis revealed varied splicing sensitivity of Esrp targets upon loss of Esrp1 or double knockout. This may explain the progressive phenotypes seen in Esrp knockout mice, and these mice provide a unique genetic tool to evaluate functional consequences of epithelial splicing events in vivo.

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RNAseq of livers from WT mice or livers with Rbfox2 knockout fed CD

Project description:Rbfox2 is required for maintaining hepatic metabolic homeostasis, here we carry out RNAseq to of WT and aged matched littermate Rbfox2 knockout mice under high fructose in order to determine changes in alternative splicing and mRNA expression caused by knockout of this splicing factor in this metabolic environment.

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Project description:Study of the RNA splicing in THUMPD2 knockout HeLa S3 cells

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Project description:Study of the RNA splicing in METTL4 knockout HeLa S3 cells

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RNAseq of livers from WT mice or livers with Rbfox2 knockout fed CD with 30% (w/v) Fructose in the drinking water

Project description:Rbfox2 is required for maintaining hepatic metabolic homeostasis. Here we carry out RNAseq to of WT and aged matched litter mate Rbfox2 knockout mice under CD or HFD in order to determine changes in alternative splicing and mRNA expression caused by knockout of this splicing factor in the context of metabolic health and disease.

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Total RNA sequencing of mouse LSK cells in E14.5 fetal liver

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