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MeRIP-seq analysis for CSTF2 knockout in HUH7 and PLC/PRF/5 cell lines

ABSTRACT: MeRIP-seq analysis for CSTF2 knockout in HUH7 and PLC/PRF/5 cell lines

PROVIDER: PRJNA1116537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29181126_1.fastq.gz	Fastqsanger.gz
	SRR29181126_2.fastq.gz	Fastqsanger.gz
	SRR29181127_1.fastq.gz	Fastqsanger.gz
	SRR29181127_2.fastq.gz	Fastqsanger.gz
	SRR29181128_1.fastq.gz	Fastqsanger.gz

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MeRIP-seq analysis for CSTF2 knockout in HUH7 and PLC/PRF/5 cell lines

Project description:CSTF2 has been shown to have a certain oncogenic effect in hepatocellular carcinoma, but its mechanism remains unclear. Previous studies have suggested that CSTF2 can shorten the 3' untranslated region (3'UTR) of target genes. Considering that the 3'UTR contains numerous m6A modification sites, we hypothesize that CSTF2 may regulate mRNA m6A modification. We performed MeRIP-seq analysis to investigate the changes in m6A modification in CSTF2 knockout HUH7 and PLC/PRF/5 cell lines.

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Project description:The role and mechanism of CSTF2 in hepatocellular carcinoma are unclear. We constructed CSTF2 knockout HUH7 and PLC/PRF/5 cells to analyze the function of CSTF2 in liver cancer and the downstream gene changes it induces.

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PLC/PRF/5 cells ChIP on CHIP

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Identifying CSTF2-bound RNA Sequences in HUH7 Cells using RIP-seq

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Expression data from hepatitis E virus inoculated PLC/PRF/5 cells

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