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Nervonic acid improves the fat transplantation by promoting adipogenesis and angiogenesis

ABSTRACT: Nervonic acid improves the fat transplantation by promoting adipogenesis and angiogenesis

PROVIDER: PRJNA1120041 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29285610_1.fastq.gz	Fastqsanger.gz
	SRR29285610_2.fastq.gz	Fastqsanger.gz
	SRR29285611_1.fastq.gz	Fastqsanger.gz
	SRR29285611_2.fastq.gz	Fastqsanger.gz
	SRR29285612_1.fastq.gz	Fastqsanger.gz

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Nervonic acid improves the fat transplantation by promoting adipogenesis and angiogenesis

Project description:Adipose tissue engraftment has become a promising strategy for reconstruction in the field of regenerative surgery. However, there are significant challenges such as reabsorption of 50–90% of the transplanted fat or cyst formation due to fat necrosis after fat transplantation. Thus, identifying novel materials or methods to improve the engraftment efficiency is crucial. Here, we investigated the effects of nervonic acid (NA) on adipogenesis and fat transplantation and its underlying mechanisms. We found that NA treatment accelerated adipogenesis through activation of the AKT/mTOR pathway and inhibition of Wnt signaling. Additionally, NA treatment enriched the expression of AKT-mTOR signaling-related genes and increased the expression of genes involved in angiogenesis and fat differentiation. Furthermore, NA effectively improved the outcome of adipose tissue engraftment. Treatment of NA reduced the resorption of transplanted fat and increased the proportion of perilipin-1+ adipocytes with a lower portion of vacuoles. Moreover, the NA-treated group showed decreased pro-inflammatory response and had more CD31+ vessel structures, which were relatively evenly distributed among viable adipocytes, facilitating the successful engraftment. In summary, we demonstrated that NA not only stimulates adipogenesis by regulating in human MSCs but improve the outcome of fat transplantation by reducing inflammation and stimulating angiogenesis. We suggest that NA could be an adjuvant strategy to support the fat transfer in regenerative surgery.

2024-08-20 | GSE269065 | GEO

Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis

Project description:Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis

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Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis [blood]

Project description:Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis [blood]

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Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis [hADSCs]

Project description:Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis [hADSCs]

| PRJNA590665 | ENA

Angiogenin Released from ABCB5+ Stromal Precursors Improves Healing of Diabetic Wounds by Promoting Angiogenesis

Project description:Angiogenin Released from ABCB5+ Stromal Precursors Improves Healing of Diabetic Wounds by Promoting Angiogenesis

| PRJNA753798 | ENA

Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis.

Project description:Adipose tissue engraftment has become a well-established therapy in plastic and reconstructive surgery used to restore age-related or injury-related soft tissue loss. However, the unpredictable absorption rates limit its further application. Some clinicians have noted that more optimal aesthetic results are achieved when botulinum toxin A (BoNTA) is applied prior to adipose tissue grafting. In the present study, we transplanted allogeneic adipose tissue treated with or without BoNTA in SD rats in vivo. We subsequently evaluated the survival rate (weight, volume, apoptosis and cellular integrity) and revascularization of the adipose tissue. The results revealed that BoNTA improved the long-term weight and volume retention of the graft, and preserved cellular integrity. BoNTA significantly increased the expression levels of CD31 and vascular endothelial growth factor (VEGF), suggesting enhanced vasodilation and endothelial cell proliferation. In vitro, adipose-derived stem cells (ASCs) were isolated, identified and induced to proliferate and differentiate with or without BoNTA. Furthermore, to evaluate the proliferative, adipogenic and angiogenic ability of the ASCs, CCK-8 assay and Oil Red O staining were conducted. Gene and protein expression levels were analyzed by RT-qPCR and western blot analysis. The results revealed that 8x10-2 U/ml BoNTA as the optimal dose increased ASC proliferation and adipogenic differentiation capacity, as well as the expression level of the key cytokine of angiogenesis. On the whole, our findings indicate that BoNTA improves adipose tissue engraftment and promotes ASC regeneration, which could benefit future clinical applications.

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Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis.

Project description:BackgroundOur previous study proved that Salvia miltiorrhiza could enhance fat graft survival by promoting adipogenesis. However, the effect of salvianolic acid B (Sal-B), the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza, on fat graft survival has not yet been investigated.ObjectiveThis study aims to investigate whether salvianolic acid B could improve fat graft survival and promote preadipocyte differentiation. The underlying mechanism has also been studied.MethodsIn vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2 and 4 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the adipogenesis and proliferative activities of salvianolic acid B were analyzed in cultured human adipose-derived stem cells (h-ADSCs) and 3T3-L1 cells to detect the mechanism by which salvianolic acid B affects graft survival.ResultsIn vivo, the weights and volumes of the fat grafts in the Sal-B-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity and more viable adipocytes were observed in the Sal-B-treated groups. In vitro, salvianolic acid B showed the ability to promote 3T3-L1 and h-ADSC proliferation and adipogenesis.ConclusionsOur in vitro experiments demonstrated that salvianolic acid B can promote the proliferation of adipose stem cells and enhance the differentiation of adipose stem cells. Simultaneously, in vivo experiments showed that salvianolic acid B can improve the survival rate of fat transplantation. Therefore, our research shed light on the potential therapeutic usage of salvianolic acid B in improving the survival rate of fat transplantation.

| S-EPMC8447755 | biostudies-literature

DIDO is necessary for adipogenesis promoting diet-induced obesity [I]

Project description:DIDO is necessary for adipogenesis promoting diet-induced obesity [I]

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Exosomes from MSC facilitate diabetic wound healing by promoting angiogenesis

Project description:Exosomes from MSC facilitate diabetic wound healing by promoting angiogenesis

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Angiogenin Released from ABCB5+ Stromal Precursors Improves Healing of Diabetic Wounds by Promoting Angiogenesis

Project description:Severe angiopathy is a major driver for diabetes associated secondary complications. Knowledge on underlying mechanisms essential for advanced therapies to attenuate these pathologies is limited. Injection of ABCB5+ stromal precursors (SPs) at the edge of non-healing diabetic wounds in a murine db/db model, closely mirroring human type II diabetes, profoundly accelerates wound closure. Strikingly, enhanced angiogenesis was substantially enforced by the release of the ribonuclease angiogenin from ABCB5+ SPs. This compensates for the profoundly reduced angiogenin expression in non-treated murine and human chronic diabetic wounds. Silencing of angiogenin in ABCB5+ SPs prior to injection significantly reduced angiogenesis, reduced numbers of M2 macrophages and delayed wound closure in diabetic db/db mice implying an unprecedented key role for angiogenin in tissue regeneration in diabetes. These data hold significant promise for further refining SPs-based therapies of non-healing diabetic foot ulcers and other pathologies with impaired angiogenesis.

2021-08-12 | GSE181881 | GEO

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