Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Low-density Lipoprotein Regulates Intestinal Stem Cell Homeostasis via PPAR Pathway

ABSTRACT: Low-density Lipoprotein Regulates Intestinal Stem Cell Homeostasis via PPAR Pathway

PROVIDER: PRJNA1120831 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Low-density Lipoprotein Regulates Intestinal Stem Cell Homeostasis via PPAR Pathway

Project description:Epidemiological studies have highlighted a strong association between hyperlipidemia and an increased risk of cancer in the gut. Intestinal stem cells (ISCs) have been demonstrated as the cells of origin for tumorigenesis in the gut. However, the impact of hyperlipidemia on ISC homeostasis remains unclear. Here, we show that hyperlipidemia induced by low-density lipoprotein receptor (Ldlr) deficiency enhances ISC proliferation in vivo. Additionally, LDL treatment impairs organoid survival but increases ISC stemness ex vivo, as evidenced by the formation of poorly differentiated spheroid and higher ISC self-renewal capacity. Mechanistically, LDL treatment activates PPAR pathways, and pharmacological inhibition of PPAR and its downstream targets, including CPT1A and PDK4, mitigates the effect of LDL on ISCs. Furthermore, although Ldlr-/- intestines exhibit increased susceptibility to irradiation-induced crypt damage, they regenerate completely within a similar timeframe as controls. These findings demonstrate that hyperlipidemia modulates ISC homeostasis, providing new insights into the mechanism linking hyperlipidemia with tumorigenesis in the gut.

2024-12-05 | GSE269253 | GEO

Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

2012-05-22 | GSE32145 | GEO

Oxidized Low-Density Lipoprotein drives dysfunction of the liver lymphatic system

Project description:Oxidized Low-Density Lipoprotein drives dysfunction of the liver lymphatic system

| PRJNA659994 | ENA

Sex differences in the expression and function of hepatic very low-density lipoprotein receptor during protein deficiency in mice

Project description:Very low-density lipoprotein (VLDL) receptor (VLDLR), a member of the low-density lipoprotein receptor family, is responsible for VLDL uptake in peripheral tissues. We reported that significant increase in hepatic VLDLR levels following protein restriction in male mice does not contribute to hepatic fat accumulation, indicating that VLDLR may have hitherto unknown functions. Here, we used RNA-sequencing analysis of liver samples to analysis the effects of protein restriction on hepatic VLDLR in male and female mice.

2024-02-27 | GSE259313 | GEO

The difference of microRNA profiles among control, normal high density lipoprotein-treated and proflammatory high density lipoprotein-treated endothelial cells

Project description:The difference of microRNA profiles among control, normal high density lipoprotein-treated and proflammatory high density lipoprotein-treated endothelial cells

| PRJNA659040 | ENA

The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice

Project description:The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice

| PRJEB29436 | ENA

Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

Project description:Retinal gene expression was assessed from postnatal day (P) 8 old low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice with a Illumina mouse-WG6 expression BeadChip. RNA was extracted from the retinas of P8 Lrp5 knockout and wild type mice (n=3 each group). Total RNA (1ug each) were reverse transcribed, followed by a single in vitro transcription amplification to incorporate biotin-labeled nucleotide, and subsequent hybridization and staining with strepatavidin-Cy3 according to the manufacturer’s instructions.

2012-05-21 | E-GEOD-32145 | biostudies-arrayexpress

genes/pathways underlying lipoprotein homeostasis

Project description:The aim of this study was to identify novel genes and pathways underlying lipoprotein homeostasis. The liver expression profile of mild hyperlipidemic E3L transgenic mice was compared to that of the wild-type B6 mice. Keywords: parallel sample

2003-11-10 | GSE828 | GEO

Low-Density Lipoprotein Receptor fuels the cellular cholesterol-mTORC1 axis required for CD8+ T cell activation

Project description:In this study, the contribution of cholesterol uptake by the low-density-lipoprotein receptor (LDLR) in the metabolic reprogramming of activated T cells was investigated at proteome level by mass-spectrometry-assisted shotgun proteomics.

2022-09-20 | PXD030365 | Pride

A sexually dimorphic hepatic cycle of very low density lipoprotein uptake and assembly

Project description:A sexually dimorphic hepatic cycle of very low density lipoprotein uptake and assembly

| PRJNA1022779 | ENA