Project description:The purpose of this study was to reveal the differences in transcriptional and metabolic levels between osteosarcoma 2D cells, 3D cells, 3D cell printed tissue, isolated tissue, and transplanted tumor in vivo
2022-12-28 | GSE201050 | GEO
Project description:Marine biofilms on 3D-printed materials
Project description:Nuclear LASP-1 has a direct correlation with the overall survival of breast cancer patients. Gene expression analysis of MCF7 human breast cancer cells cultured in 3D-Matrigel was performed. Up regulation of cell junction proteins, extracellular matrix proteins and down regulation of MMP 9 and 2 were observed. This corroborates well with the involvement of LASP-1 in cell migration and chemotaxis. Non-silencing (control) and LASP-1 knock down MCF7 cells were cultured on 3D-Matrigel, total RNA was extracted and anlyzed - 4 biological replicates each. The first biological replicate was done as a pilot. Biological replicates 2-4 were run as a second set after pilot.
