Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1123870 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29409509_1.fastq.gz | Fastqsanger.gz | |||
| SRR29409509_2.fastq.gz | Fastqsanger.gz | |||
| SRR29409510_1.fastq.gz | Fastqsanger.gz | |||
| SRR29409510_2.fastq.gz | Fastqsanger.gz | |||
| SRR29409511_1.fastq.gz | Fastqsanger.gz |
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Project description:This project aims to analyze rice plasma membrane proteins related to resistance against rice blast infection. We extracted rice plasma membrane proteins before and after M.oryzae infection for 24h, and then used trypsin to digest and iTRAQ to label the peptides, HPLC-MS/MS was used to seperate and identify peptides. 1.1/0.909 fold change with p-value < 0.05 was used as threshold for differentially expressed proteins. 2,977 proteins were identified and 951 of which were found to be responsive to resistance against M. oryzae. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein interaction network showed that plenty of proteins were involved in vesicle trafficking with obvious functional tendencies towards transport, vesicle-mediated transport, secretion, endocytosis and phagosome. 10 DEPs were validated at transcript level, and a SNARE protein named NPSN (novel plant-specific SNARE)13 actively responded to M. oryzae infection, and it contributed to rice blast resistance and mainly located at PM.
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Project description:WRKY45-overexpressing (WRKY45-ox) rice plants are extremely resistant to rice blast and bacterial leaf-blight diseases. To search for the genes that are potentially responsible for the strong resistance, we performed genome-wide gene expression analysis in WRKY45-ox rice. A microarray analysis using Agilent rice oligo microarray for approximately 42,000 rice genes identified 1,664 genes that are upregulated in WRKY45-ox rice compared with non-transformant rice cv. Nipponbare (NB). Analysis of the genes with altered expression in WRKY45-ox rice by a Gene ontology program (Agri GO; http://bioinfo.cau.edu.cn/agriGO/) revealed that several genes in the biosynthetic pathway for diterpenoid phytoalexins, for which anti-blast fungus activities have been reported, were upregulated in WRKY45-ox rice.
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Project description:In rice, a number of resistance (R) genes that counter the blast fungus, Magnaporthe oryzae, have been cloned, but the mechanism by which they trigger disease resistance remains elusive. This is in part due to a lack of comprehensive transcriptome analyses during the resistance or disease progression. Here, we monitored the gene expression profiling in rice during its interactions with Magnaporthe by time-series transcriptome analyses. Distinct from previous studies, we focused on early infection stages within 24 hours post-inoculation. A comparison of those expression changes revealed a general difference in gene expression kinetics between compatible and incompatible interactions, and pointed to that the time when the pathogen just establishes its penetration into rice cells is a key point for the expressional changes. Such conclusions originated from the R gene Pid3-mediated immune responses were validated in the R gene Pi9-mediated responses, suggesting common molecular processes are shared by different R-mediated blast immunity. Our data highlighted the role of jasmonic acid (JA)-triggered signaling pathway (JA pathway) in the hormone signaling network for rice blast resistance. We confirmed that both exogenous and endogenous JA can induce the expression of many defense-related components revealed in above transcriptomic analyses and proved that the knock-down of OsCOI1 which encodes a JA receptor may deprive rice of the blast resistance mediated by R genes. Therefore, it is concluded that JA pathway plays an essential role in the establishment of blast resistance by modulating the expression of other defense-related components.
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Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pish or its mutant, pish) were inoculated with rice blast fungus, Kyu77-07A, carrying avirulence gene, AvrPish. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).
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Project description:WRKY45-overexpressing (WRKY45-ox) rice plants are extremely resistant to rice blast and bacterial leaf-blight diseases. To search for the genes that are potentially responsible for the strong resistance, we performed genome-wide gene expression analysis in WRKY45-ox rice. A microarray analysis using Agilent rice oligo microarray for approximately 42,000 rice genes identified 1,664 genes that are upregulated in WRKY45-ox rice compared with non-transformant rice cv. Nipponbare (NB). Analysis of the genes with altered expression in WRKY45-ox rice by a Gene ontology program (Agri GO; http://bioinfo.cau.edu.cn/agriGO/) revealed that several genes in the biosynthetic pathway for diterpenoid phytoalexins, for which anti-blast fungus activities have been reported, were upregulated in WRKY45-ox rice. Nipponbare rice (Oryza sativa) and WRKY45-ox rice were analyzed in three biological replicates.
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