Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RIAN-seq]

ABSTRACT: Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RIAN-seq]

PROVIDER: PRJNA1125034 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29445008_1.fastq.gz	Fastqsanger.gz
	SRR29445008_2.fastq.gz	Fastqsanger.gz
	SRR29445009_1.fastq.gz	Fastqsanger.gz
	SRR29445009_2.fastq.gz	Fastqsanger.gz
	SRR29445010_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 64

Similar Datasets

Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RNA-seq]

Project description:R-loop is abundant triplex nucleic strand in organism, but many physiological and pathological functions remain to be fully understood, owing to a lack of robust and high-resolution methods for genome-wide detection. In this study, we develop R-loop identification assisted by nuclease and high-throughput sequencing (RIAN-seq), a method to identify and quantify R-loop stoichiometry at affinity-independent enrichment. We reveal that RIAN-seq has ultrahigh sensitivity, accuracy and reliability for identifying R-loop sites, compared with all existing methods. In addition, RIAN-seq enables capturing previously unidentified clustered R-loop sites with distinct patterns. In sum, we reveal that RIAN-seq can not only quantitatively dissect the R-loop landscape at unprecedented resolution, but also provide an unbiased and convenient method for R-loop biology.

2025-03-18 | GSE270122 | GEO

Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RIAN-seq]

2025-03-18 | GSE270120 | GEO

Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [HEK293T]

2025-03-18 | GSE254470 | GEO

Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [mESC]

2025-03-18 | GSE254469 | GEO

Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RNA-seq]

Project description:Genome-wide profiling of R-loop by affinity-independent and nuclease-based method [RNA-seq]

| PRJNA1125036 | ENA

Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [mESC]

Project description:Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [mESC]

| PRJNA1070626 | ENA

Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [HEK293T]

Project description:Affinity-independent and nuclease-based strategy to profile R-loop genome-wide [HEK293T]

| PRJNA1070625 | ENA

BisMapR: a strand-specific, nuclease-based method for genome-wide R-loop detection

Project description:We have developed an antibody-independent and strand-specific R-loop detection strategy, BisMapR, that combines nuclease-based R-loop isolation with non-denaturing bisulfite chemistry to produce high-resolution, genome-wide R-loop sequencing maps.

2021-01-29 | GSE160578 | GEO

BisMapR: a strand-specific, nuclease-based method for genome-wide R-loop detection

Project description:BisMapR: a strand-specific, nuclease-based method for genome-wide R-loop detection

| PRJNA673629 | ENA

mDRIP-seq: a high-throughput method for R-loop mapping and quantitative assessment.

Project description:Current methods for R-loop mapping need to perform DNA:RNA immunoprecipitation for each sample individually, with consequent limitations in throughput. Here, we develop and validate mDRIP-seq, a multi-sample barcoding and pooling method for R-loop mapping. We show mDRIP-seq performs equivalently as conventional methods, but with the merits of high throughput and cost-efficiency. We also show the simplicity of mDRIP-seq for relative and absolute quantitation of genomic R-loop fractions for multiple samples. Together, mDRIP-seq is a high-throughput and cost-efficient method for R-loop mapping and quantitative assessment and can be widely applied to large-scale dynamic profiles of these important structures for diverse organisms.

2024-06-12 | GSE246423 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data