Project description:Ten cattle have been challenged with two Lumpy Skin Disease Virus (LSDV). They were sampled for whole blood immediately before (pre) and three and seven days after (post) infection challenge with two virus strains (H vs. O). The whole RNA-sequencing was done, and 150bp paired reads were assembled as the transcriptome. It was then computationally analyzed to find the differentially expressed genes (DGE) that enrich the gene ontology (GO) terms and KEGG pathways. Depending on the challenged LSDV strain, they influence the host response differently.
Project description:Lumpy Skin Disease (LSD) is an endemic viral infection in Africa, newly emerging in Europe and becoming a global threat. There is host variation in response to infections in field studies and under carefully controlled artificial infections. It’s sometimes around 50% in clinical trials. This study aims to understand the mechanisms underlying this diversity and find the determinants to distinguish between susceptible (recovered) and resistant (non-recovered) animals through Differential Gene Expression (DGE) analysis. Five approximately 6-month-old male Holstein bulls were experimentally infected with LSDV. RNA sequencing was done for whole blood samples collected five days prior (pre-infection) to 3, 7, and 15 days after the infection (post-infection) challenge. After the challenge, two animals recovered, and three did not.
