Project description:This SuperSeries is composed of the following subset Series: GSE12775: Karyotype analysis of S. cerevisiae chromosome replacement lines - a-type cells GSE12776: Karyotype analysis of S. cerevisiae chromosome replacement lines - alpha-type cells Refer to individual Series
Project description:The S. cerevisiae hybrid karyotypes were analyzed by array-CGH to identify small regions of duplication or homeologous chromosomal exchange occurring during the strain construction. alpha-type cells. S. cerevisiae vs. Chromosome replacement lines. Biological replicates: 1 control (S. bayanus), 11 Chromosome replacement lines, independently grown and harvested. Two replicate per array.
Project description:The S. cerevisiae hybrid karyotypes were analyzed by array-CGH to identify small regions of duplication or homeologous chromosomal exchange occurring during the strain construction. a-type cells. S. cerevisiae vs. Chromosome replacement lines. Biological replicates: 1 control (S. bayanus), 11 Chromosome replacement lines, independently grown and harvested. Two replicate per array.
Project description:Hematopoietic malignancies are frequently characterized by karyotypic abnormalities. The development of targeted drugs has been pioneered with compounds against gene products of fusion genes caused by chromosomal translocations. While polysomies are equally frequent as translocations, for many of them we are lacking therapeutic approaches aimed at synthetic lethality. Here, we report two new cell lines, named MBU-7 and MBU-8, that differ in complete trisomies of chromosome 18, a partial trisomy of chromosome 7 and a tetrasomy of the p-arm of chromosome 8, but otherwise share the same mutational pattern and complex karyotype. Both cell lines are divergent clones of U-937 cells and have the morphology and immunoprofile of monocytic cells. The distinct karyotypic differences between MBU-7 and MBU-8 are associated with a difference in the specific response to nucleoside analogues. Taken together, we propose the MBU-7 and MBU-8 cell lines described here as suitable in vitro models for screening and testing vulnerabilities that are associated with the disease-relevant polysomies of chromosome 7, 8 and 18.
Project description:Transcription factors are speculated to play crucial roles in adaptive evolution. Here we investigated how essential transcription factors (eTFs) change using ortholog replacement assays. Several orthologous eTFs from other yeast species could not fully complement the mutants in Saccharomyces cerevisiae, suggesting that these eTFs have changed their functions or interactions to become incompatible. We further characterized TFIIIC, a protein complex assisting RNA polymerase III transcription, that exhibits complete or partial incompatibility in several subunits. In the orthologous Tfc7-replacement line, the binding of TFIIIC to tRNA genes is reduced, but the abundance of tRNAs is not severely affected. However, Tfc7-replacement cells often mis-segregate chromosomes during mitosis and their fitness is further reduced in the spindle checkpoint mutant. Chromatin immunoprecipitation experiments showed that unstable TFIIIC binding results in defective cohesion loading, leading to chromosome mis-segregation. Swapping the highly divergent C-terminal domain of Tfc7 orthologs rescues its interaction with Tfc1 and cell fitness, suggesting that incompatibility is caused by altered interactions between complex subunits. Our results reveal separatable essential functions of a well-studied protein complex.
