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Cashmere goat gastrointestinal microorganisms

ABSTRACT: cashmere goat gastrointestinal microorganisms Raw sequence reads

PROVIDER: PRJNA1132486 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29762410_1.fastq.gz	Fastqsanger.gz
	SRR29762410_2.fastq.gz	Fastqsanger.gz
	SRR29762411_1.fastq.gz	Fastqsanger.gz
	SRR29762411_2.fastq.gz	Fastqsanger.gz
	SRR29762412_1.fastq.gz	Fastqsanger.gz

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A single-cell transcriptome atlas during Cashmere goat hair follicle morphogenesis

Project description:Cashmere, also known as soft gold, is produced from secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stage fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also successfully characterized. Based on cell lineage inference, we revealed detailed transcriptional gene expression profiles during dermal and epidermal lineage cell fate decisions. These works together delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.

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A Microarray-Based Analysis Reveals That A Short Photoperiod Promotes Hair Growth in the Arbas Cashmere Goat

Project description:we evaluated the Arbas Cashmere goat, a species that is especially used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclical cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Based on assessments of tissue sections, it was observed that the short photoperiod significantly induced cashmere growth. A comparison of the differences in gene expression between the short photoperiod and the natural conditions using gene chip technology supported this conclusion. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and it was found that these genes are involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Based on their similar weights, health conditions and good reproductive performance in a group of the same strain, we chose 62-year-old female adult Arbas Cashmere goats as our research specimens and randomly divided them into a test group (T1, T2, T3) and a control group (C1, C2, C3).The goats in the test group were grazed, fed, and allowed to drink from 9:30-16:30 daily, and they were housed in a dark shed with good air conditions for the rest of the time. The temperature in the shed was 1 degree less than that outside, and the opacity was controlled at approximately 0.1 lux. The control group was grazed in natural conditions. We used regular management to care for all of the goats.

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