Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1132543 | ENA |
REPOSITORIES: ENA
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Project description:Self-inhibition of pollen tubes plays a key role in SI, but the underlying mechanism in Camellia oleifera is poorly understood. Collection of secreted proteins from Camellia oleifera pollen tubes and ovaries for high-throughput sequencing.
2023-03-11 | PXD035406 | Pride
Project description:We report the expression analysis of seed kernel in Camellia oleifera cultivars. In total 221 cultivars are sequenced by the Illumina sequencing experiments to obtain the gene expression profiles.
2021-12-12 | GSE190644 | GEO
Project description:Floral organs are extremely sensitive to stress during anthesis and lead to severe yield loss. Rice anthers and pollinated pistils of two cultivars with contrasting tolerance to heat and drought stress under variable conditions, including control, heat, combined heat and drought stress, were used to explore gene expression pattern in male and female reproductive organs during anthesis under control and stress conditions. More gene regulation was induced by combined drought and heat stress than heat in anthers of both cultivars. N22 showed less regulation under combined stress than Moroberekan. The overlap of regulated genes between two cultivars was rather low, indicated the distinct molecular stress responses. We used whole genome microarrays to explore gene expression pattern and molecular mechanisms in male and female reproductive organs during anthesis under control and stress conditions in two rice cultivars, sought to identify the key transcripts that play roles in inducing heat and drought tolerance during reproduction in rice.
2015-05-31 | GSE57154 | GEO
Project description:To identify the important genetic resources of tea oil accumulation and quality formation in Camellia oleifera, an important woody edible oil tree native to Southern China, we have designed and customized an expression profile chip of C. oleifera with 8×60 K on the basis of transcriptome sequencing of multiple tissue samples including kernels, roots, and leaves from multiple varieties. we used the mcroarrays to determine the gene expressions in kernel development of C. oleifera elite varieties'Huashuo' , 'Huaxin' , 'Huajin' and 'Jujian' respectively. Microarray results indicated a total of 10710 gene probes showed stable differential expression in the comparation of August vs June and 9987 in the comparation of October vs August. PATHWAY enrichment results of DEGs indicated that the oil synthesis and accumulation occured in the whole kernel development of C. oleifera, but were mainly concentrated from the nutrition high-speed synthesis period to the seed mature period, which was consistent with the variation trend of oil content and fatty acide composition in C. oleifera kernel development.
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Project description:To dissect the molecular mechanisms underlying drought tolerance (DT) in rice, transcriptome differences of a DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing. Results revealed a differential constitutive gene expression prior to stress and distinct global transcriptome reprogramming among three genotypes under time-series drought stress, consistent with their differential genotypes and DT phenotypes. DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing.The drought stress treatment was started by withholding water at the tillering stage. The days were counted after the AWC in the soil reached 20% to allow drought measurements at precisely determined intervals, and the soil water content reached 15%, 10% and 7.5% after 1d, 3d and 4d drought treatment, respectively.Three top leaves for each sample were harvested for each genotype under 1d and 3d drought stress and control conditions. All samples were immediately frozen in liquid nitrogen and stored at -80C and then for transcriptome sequencing.
2014-12-30 | E-GEOD-57950 | biostudies-arrayexpress
Project description:To dissect the molecular mechanisms underlying drought tolerance (DT) in rice, transcriptome differences of a DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing. Results revealed a differential constitutive gene expression prior to stress and distinct global transcriptome reprogramming among three genotypes under time-series drought stress, consistent with their differential genotypes and DT phenotypes.
2014-12-30 | GSE57950 | GEO
Project description:Integrated breeding strategies are used to increase both the yield potential and stability of crops. Most of these approaches have a direct genetic basis. The utility of epigenetics in breeding to improve complex traits such as yield and stress tolerance is not clear. A better understanding of the status of the epigenome and its contribution to the agronomic performance would help in developing strategies to incorporate the epigenetic component of complex traits in breeding,Starting from isogenic canola lines, epilines were generated by selecting recursively during three generations for lines with a higher energy use efficiency and drought tolerance. These epilines were more energy use efficient, drought tolerant, high nitrogen use efficient, and higher yielding under suboptimal conditions. Moreover, these characteristics were transgenerational inheritable. Transcriptome comparison with a line selected for energy use efficiency only revealed common differentially expressed genes related to the onset of signaling events regulating stress tolerance. Genes related to salt, osmotic, abscisic acid and drought were specifically differentially expressed in the drought tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine 4 of histone 3, supports the energy use efficient and drought tolerant phenotype by facilitating transcription of the genes that are found to be differentially expressed.From these results it can be concluded that the epigenome can be shaped by selection to increase yield and stress tolerance. This acquired knowledge will support further development of strategies to incorporate epigenetics in breeding. SRA study accession: SRP052946, Bioproject: PRJNA273932. Two canola (Brassica napus L. spp. oleifera (Metzg) Sinsk. f. biennis) epilines with low respiration and high NAD(P)H content were selected. Grinded leaf material from 26 day-old control and both epilines was collected in triplicate and served as input for deep sequencing on Illumina GAIIx.
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Project description:To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and dry conditions, respectively. In total, 4,063,419 high quality sequencing contigs, 175,649 transcripts and 75,265 unigenes were obtained in this study. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment.
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