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Quantification of basal RNA mutation rate in the repRNA-v4 EGFP region

ABSTRACT: Quantification of basal RNA mutation rate in the repRNA-v4 EGFP region

PROVIDER: PRJNA1133799 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29759462.fastq.gz	Fastqsanger.gz
	SRR29759463.fastq.gz	Fastqsanger.gz
	SRR29759464.fastq.gz	Fastqsanger.gz
	SRR29759465.fastq.gz	Fastqsanger.gz
	SRR29759466.fastq.gz	Fastqsanger.gz

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Quantification of basal RNA mutation rate in the repRNA-v4 EGFP region

Project description:REPLACE was engineered from an orthogonal alphaviral RNA replication system. It generates a large, continuously diversified library of replicative RNAs through a replicase-limited mode of replication and inducible mutagenesis. We analyzed the mutation frequency at Day0 (IVT RNA), Day1, Day3, Day7, Day14, Day21 and DNA plasmid to estimate the RNA mutation rate. This data was used to guide the construction of RNA mutant libraries.

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