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Coumaric acid stressed soil

ABSTRACT: Coumaric acid stressed soil Raw sequence reads

PROVIDER: PRJNA1135263 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29810932.fastq.gz	Fastqsanger.gz
	SRR29810933.fastq.gz	Fastqsanger.gz
	SRR29810934.fastq.gz	Fastqsanger.gz
	SRR29810935.fastq.gz	Fastqsanger.gz
	SRR29810936.fastq.gz	Fastqsanger.gz

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Project description:We aimed to study how production of p-coumaric acid, a precursor of multiple secondary aromatic metabolites, influences the cellular metabolism of Saccharomyces cerevisiae. We evaluated the growth and p-coumaric acid production in batch and chemostat cultivations and analyzed the transcriptome and intracellular metabolome during steady state in low- and high-producers of p-coumaric acid in two strain backgrounds, S288c or CEN.PK. For analysis of the differential gene expression, we did pairwise comparisons between the optimized and non-optimized strains for p-CA production: CEN.PK strains (ST4288 and ST4408) and the S288c strains (ST4353 and ST4397). Transcriptome analysis showed that the CEN.PK strain was less affected by engineering towards higher p-CA production than the S288c strain, as the number of significantly up-/down-regulated genes was correspondingly 652 and 1927 amongst others, strain S288c had downregulations in gene sets involved in amino acid and protein biosynthesis. This suggests that CEN.PK may be a better platform strain for production of aromatic compounds than the S288c strain.

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