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Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin

ABSTRACT: Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin

PROVIDER: PRJNA1135480 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29819335_1.fastq.gz	Fastqsanger.gz
	SRR29819335_2.fastq.gz	Fastqsanger.gz
	SRR29819336_1.fastq.gz	Fastqsanger.gz
	SRR29819336_2.fastq.gz	Fastqsanger.gz
	SRR29819337_1.fastq.gz	Fastqsanger.gz

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Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin

Project description:Airway epithelial cells represent the first line of defense against respiratory pathogens. Flagellin drives the motility of many mucosal pathogens and has been suggested as an immune enhancing adjunctive therapeutic in infections of the airways. This study leveraged single-cell RNA sequencing to determine cell-specific effects of flagellin in primary human bronchial epithelial cells growing in air-liquid interface. Seven cell clusters were identified, including ciliated cells, ionocytes and several states of basal and secretory cells, of which only inflammatory basal cells and inflammatory secretory cells demonstrated a proportional increase in response to flagellin. Inflammatory secretory cells showed evidence of metabolic reprogramming toward aerobic glycolysis, whilst in inflammatory basal cells transcriptome profiles indicated enhanced oxidative phosphorylation. Inhibition of mTOR prevented the shift to glycolysis and reduced inflammatory gene transcription specifically in inflammatory secretory cells. This data demonstrates the functional heterogeneity of the human airway epithelium upon exposure to flagellin.

2024-09-18 | GSE272188 | GEO

Innate immune responses by bronchial epithelial cells exposed to the bacterial component flagellin

Project description:RNAseq was used to measure the transciptional respose of human bronchial epithelium (HBE) to the bacterial component flagellin with particular emphasis on innate immunity and metabolism.

2021-01-13 | GSE164704 | GEO

flagellin

Project description:Flagellin diversity

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Innate immune responses by bronchial epithelial cells exposed to the bacterial component flagellin

Project description:Innate immune responses by bronchial epithelial cells exposed to the bacterial component flagellin

| PRJNA691599 | ENA

An unorthodox flagellin paralog integrates cell cycle and flagellar assembly cues for flagellin translation (Tn-seq)

Project description:How cellular checkpoints couple the orderly assembly of macromolecular machines with cell cycle progression is poorly understood. The alpha-proteobacterium Caulobacter crescentus assembles a single polar flagellum during each cell cycle. We discovered that expression of multiple flagellin transcripts is licensed by a translational checkpoint responsive to a dual input signal: a secretion-competent hook-basal-body (HBB) structure and a surge in the FlaF secretion chaperone during cytokinesis, instructed by the cell cycle circuitry. We find that the unorthodox FljJ flagellin, one of six flagellin paralogs, acts as checkpoint linchpin, binding both FlaF and the FlbT translational regulator. FljJ recruits FlbT to inhibit translation at the 5’ untranslated region in other flagellin transcripts before HBB assembly. Once FlaF is synthesized and stabilized, it directs FljJ secretion through the HBB, thereby separating FlbT from its co-activator and relieving translational inhibition. The FlbT/FlaF pair is wide-spread and functional properties are conserved in alpha-proteobacteria, including pathogens.

2020-10-27 | GSE153949 | GEO

An unorthodox flagellin paralog integrates cell cycle and flagellar assembly cues for flagellin translation (RNA-seq)

2020-10-27 | GSE153858 | GEO

Pig-specific skin transcriptional responses after intradermal administration of flagellin

Project description:The study aimed at defining the skin innate responses after intradermal injection of flagellin. For this purpose, pigs were immunized by intradermal route with flagellin and H1N1 antigen. Skin was then sampled for microarray analyses.

2016-01-31 | GSE72256 | GEO

mpk6 - Flagellin response in mpk mutants

Project description:au-09-01_mpk_flagellin_edu - mpk6 - Investigate flagellin mpk dependent genes - Comparision between mpk6 and Col-0 seedlings. Keywords: gene knock out

2012-01-01 | GSE23191 | GEO

bak1bkk1 - Flagellin response in mpk mutants

Project description:au-09-01_mpk_flagellin_edu - bak1bkk1 - Investigate flagellin mpk dependent genes by comparision between bak1bkk1 and col-0. Keywords: gene knock out

2012-01-01 | GSE23185 | GEO

Analysis of microRNA expression in murine BMDCs activated by flagellin

Project description:MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. However, the role of miRNAs in inflammation induced by flagellin (ligand of TLR5) has not been fully determined, Here, we identified differentially expressed miRNAs in murine bone marrow-derived dendritic cells (BMDCs) between flagellin (FliC) treatment and medium alone using miRNA microarray. And we further evaluated the role of miRNAs in inflammation induced by flagellin.

2021-11-13 | GSE188656 | GEO

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