Project description:Biological bases for sexual differences in the brain exist in a wide range of vertebrate species, including the chicken. We examined whether sexually dimorphic gene expression in the brain precedes gonadal differentiation. Using the Affymetrix GeneChip® Chicken Genome Array, we identified many female- and male-enhanced genes that are differentially expressed in sex-specific brains from stage 29 chicken embryos. We postulate that these genes have potential roles in the sexual differentiation of neural function and development in chickens.
Project description:Biological bases for sexual differences in the brain exist in a wide range of vertebrate species, including the chicken. We examined whether sexually dimorphic gene expression in the brain precedes gonadal differentiation. Using the Affymetrix GeneChip® Chicken Genome Array, we identified many female- and male-enhanced genes that are differentially expressed in sex-specific brains from stage 29 chicken embryos. We postulate that these genes have potential roles in the sexual differentiation of neural function and development in chickens. Experiment Overall Design: All analyses were performed in triplicate. The Affymetrix data from male and female brain samples at stage 29 were generated. The male and female brain samples by genetic sexing were pooled and homogenized. Pooled samples ranged from 5 to 8 embryos. 4g of total RNA from the pooled samples was used for labeling. Probe synthesis from total RNA samples, hybridization, detection, and scanning were performed according to standard protocols from Affymetrix.
Project description:The sexually dimorphic gene expression profiles are established in early embryogenesis and epigenetically perpetuated at every stage of cardiac development.
Project description:This study provides a screen of sexually dimorphic tissue-specific (brain) gene expression in fruit flies age 3, 7, and 14 days post-eclosion. This work can be used as a baseline reference point for comparing sex-specific brain gene expression levels in fruit flies under stress.
Project description:Tissue tolerance is a sexually dimorphic trait. Here, we investigated the role of BCL6 in establishing hepatic chromatin landscape promoting sexually dimorphic tissue tolerance during E.Coli infection.
Project description:The liver is one of the most sexually dimorphic organs as measured by gene expression differences. About 80% of the sexually dimorphic genes are known to be regulated by growth hormone (GH). Somatostatin (SST) inhibits the release of GH. We generated a SST-knockout mouse and analyzed the hepatic gene expression changes in both sexes.
